1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. LCC 蛋白, Unknown prokaryotic organism (His)

LCC 蛋白, Unknown prokaryotic organism (His)

目录号: HY-P702167
产品使用指南

LCC 蛋白水解角质,即植物角质层的结构聚酯。LCC 蛋白, Unknown prokaryotic organism (His) 是重组的 LCC 蛋白,由 E. coli 表达,带有 N-6*His 标签。LCC 蛋白, Unknown prokaryotic organism (His) 全长 258 个氨基酸。

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描述

LCC proteolyzes cutin, the structural polyester of plant cuticles. LCC Protein, Unknown prokaryotic organism (His) is the recombinant LCC protein, expressed by E. coli , with N-6*His labeled tag. The total length of LCC Protein, Unknown prokaryotic organism (His) is 258 a.a., .

研究背景

LCC 蛋白在细胞过程中发挥着关键作用,因为它催化角质的水解,角质是一种对植物角质层结构组成至关重要的聚酯。各种研究证明,LCC 对对硝基苯酚连接的脂肪族酯(pNP-脂肪族酯)表现出酯酶活性,表明对短链底物(最多 C4)的偏好。有趣的是,LCC 显示出无法水解橄榄油。此外,这种多功能酶具有降解全球最流行的聚酯塑料聚对苯二甲酸乙二醇酯的能力,并且还可以解聚聚(ε-己内酯)(PCL)(一种合成脂肪族可生物降解聚酯)。LCC 的多方面底物特异性强调了其在多种聚酯酶促分解中的重要性,突出了其在天然和合成聚合物降解过程中的潜在应用。

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

G9BY57 (S36-Q293)

基因 ID

/

蛋白结构
N-term
6*His
LCC (S36-Q293)
Accession # G9BY57
C-term
同用名
Leaf-branch compost cutinase; LC-cutinase; LCC; PET-digesting enzyme; Poly(ethylene terephthalate) hydrolase; PET hydrolase; PETase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LCC Protein, Unknown prokaryotic organism (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LCC Protein, Unknown prokaryotic organism (His)
目录号:
HY-P702167
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