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  3. LIV-1/SLC39A6 蛋白, Human (Baculovirus, His)

LIV-1/SLC39A6 蛋白, Human (Baculovirus, His)

目录号: HY-P700463
产品使用指南

LIV-1/SLC39A6 是一种锌流入转运蛋白,可调节锌稳态并诱导上皮间质转化 (EMT)。与SLC39A10配合使用,可促进细胞锌吸收并触发EMT。LIV-1/SLC39A6 蛋白, Human (Baculovirus, His) 是重组的 LIV-1/SLC39A6 蛋白,由 Sf9 insect cells 表达,带有 C-6*His 标签。LIV-1/SLC39A6 蛋白, Human (Baculovirus, His) 全长 297 个氨基酸,分子量为 35.0 kDa。

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描述

LIV-1/SLC39A6 is a zinc influx transporter that regulates zinc homeostasis and induces epithelial-mesenchymal transition (EMT). Used in conjunction with SLC39A10, it can promote cellular zinc absorption and trigger EMT. LIV-1/SLC39A6 Protein, Human (Baculovirus, His) is the recombinant human-derived LIV-1/SLC39A6 protein, expressed by Sf9 insect cells , with C-6*His labeled tag. The total length of LIV-1/SLC39A6 Protein, Human (Baculovirus, His) is 297 a.a., with molecular weight of 35.0 kDa.

研究背景

LIV-1/SLC39A6 是一种锌流入转运蛋白,可复杂地调节锌稳态并有助于诱导上皮间质转化 (EMT)。从功能上讲,当与 SLC39A10 形成异二聚体时,该复合物会介导细胞锌的摄取,成为 EMT 的关键触发因素。SLC39A10-SLC39A6 异二聚体不仅控制 NCAM1 磷酸化,还影响其在 EMT 过程中整合到粘着斑复合物中。这种异二聚体复合物促进的锌流入在 GSK3B 失活中发挥着至关重要的作用,导致未磷酸化 SNAI1 的核积累,随后下调 CDH1 等粘附基因,从而促进细胞粘附的丧失。除了参与 EMT 之外,SLC39A10-SLC39A6 异二聚体通过将锌导入细胞,触发最终导致有丝分裂开始的途径,在启动有丝分裂中发挥着重要作用。此外,这种转运蛋白复合物有助于 T 细胞受体信号传导调节,并促进卵母细胞到卵子转变过程中减数分裂进展的适当锌流入。

种属

Human

表达系统

Sf9 insect cells

标签

C-6*His

蛋白编号

Q13433 (F29-W325)

基因 ID
蛋白结构
N-term
Reg1 (F29-W325)
Accession # Q13433
6*His
C-term
同用名
SLC39A6; LIV-1; ZIP6; Zinc transporter ZIP6; ZIP-6
氨基酸序列

FPQTTEKISPNWESGINVDLAISTRQYHLQQLFYRYGENNSLSVEGFRKLLQNIGIDKIKRIHIHHDHDHHSDHEHHSDHERHSDHEHHSEHEHHSDHDHHSHHNHAASGKNKRKALCPDHDSDSSGKDPRNSQGKGAHRPEHASGRRNVKDSVSASEVTSTVYNTVSEGTHFLETIETPRPGKLFPKDVSSSTPPSVTSKSRVSRLAGRKTNESVSEPRKGFMYSRNTNENPQECFNASKLLTSHGMGIQVPLNATEFNYLCPAIINQIDARSCLIHTSEKKAEIPPKTYSLQIAW

分子量

35.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LIV-1/SLC39A6 Protein, Human (Baculovirus, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LIV-1/SLC39A6 Protein, Human (Baculovirus, His)
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HY-P700463
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