LIV-1/SLC39A6 蛋白, Rat (Sf9, His)

The LIV-1/SLC39A6 protein is an important zinc influx transporter that maintains zinc balance and drives epithelial-to-mesenchymal transition (EMT). It cooperates with SLC39A10 to trigger EMT by promoting zinc uptake, regulating NCAM1 phosphorylation, and integrating it into focal adhesion complexes. LIV-1/SLC39A6 Protein, Rat (Sf9, His) is the recombinant rat-derived LIV-1/SLC39A6 protein, expressed by Sf9 , with C-His labeled tag. The total length of LIV-1/SLC39A6 Protein, Rat (Sf9, His) is 291 a.a., with molecular weight of 55-65 kDa.

LIV-1/SLC39A6蛋白是一种锌流入转运蛋白，在维持锌平衡和促进上皮细胞向间充质细胞转变中发挥着至关重要的作用。通过与 SLC39A10 形成异二聚体，它可以促进锌的摄取，从而触发上皮-间质转化 (EMT) 过程。这种异二聚体还调节 NCAM1 的磷酸化及其在 EMT 过程中整合到粘着斑复合物中。锌的流入会使 GSK3B 失活，导致 CDH1 等粘附基因下调，从而导致细胞粘附丧失。此外，SLC39A10-SLC39A6 异二聚体通过将锌导入细胞，启动导致有丝分裂开始的途径，对于启动有丝分裂至关重要。它还通过介导活化淋巴细胞中的锌摄取来调节 T 细胞受体信号传导。此外，它还调节减数分裂正常进展到中期 II (MII) 所需的锌流入，促进从卵母细胞到卵子的转变。

Rat

Sf9 insect cells

C-His

Q4V887 (L21-W311)

291733  [NCBI]

Approximately 46 kDa. The reducing (R) protein migrat es as 46 kDa in SDS-PAGE may be due to glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.