GBA/Glucosylceramidase 蛋白, Mouse (P.pastoris, His)

GBA/glucosylceramidase protein catalyzes the hydrolysis of glucosylceramide/GlcCers into free ceramide and glucose within lysosomes. It plays a key role in lipid degradation and cell membrane turnover. GBA/Glucosylceramidase Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived GBA/Glucosylceramidase protein, expressed by P. pastoris , with N-His labeled tag. The total length of GBA/Glucosylceramidase Protein, Mouse (P.pastoris, His) is 496 a.a., with molecular weight of ~57.5 kDa.

GBA/葡萄糖神经酰胺酶蛋白在溶酶体区室中充当关键酶，催化葡萄糖神经酰胺/GlcCers（例如 β-D-葡萄糖基-(1<->1')-N-acylsphing-4-enine）水解成游离神经酰胺，例如N-酰基鞘-4-烯氨酸和葡萄糖。这种酶促过程对于复合脂质的降解和细胞膜的周转至关重要。此外，GBA 通过神经酰胺的产生，在 PKC 激活的神经酰胺形成补救途径中发挥作用。该蛋白质具有转糖基化活性，通过将葡萄糖从 GlcCer 转移到胆固醇来促进胆固醇的糖基化。它显示出偏好含有单不饱和脂肪酸的 GlcCer 作为胆固醇糖基化的葡萄糖供体。在特定条件下，GBA可催化逆反应，将葡萄糖从胆固醇3-β-D-葡萄糖苷转移到神经酰胺，或水解胆固醇3-β-D-葡萄糖苷，产生葡萄糖和胆固醇。另外，GBA可以水解半乳糖神经酰胺/GalCers，例如β-D-半乳糖基-(1→1')-N-酰基鞘-4-烯氨酸，并在GalCers和胆固醇之间转移半乳糖，尽管其活性低于GlcCers。值得注意的是，木糖神经酰胺/XylCer 不能作为良好的水解底物，但它可以作为有效的木糖供体，进行木糖转移活性，形成胆固醇 3-β-D-木糖苷。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

P. pastoris

N-His

P17439 (A20-Q515)

14466  [NCBI]

AQPCIPKSFGYSSVVCVCNASYCDSLDPVTLPALGTFSRYESTRRGRRMELSVGAIQANRTGTGLLLTLQPEKKFQKVKGFGGAMTDATALNILALSPPTQKLLLRSYFSTNGIEYNIIRVPMASCDFSIRVYTYADTPNDFQLSNFSLPEEDTKLKIPLIHQALKMSSRPISLFASPWTSPTWLKTNGRVNGKGSLKGQPGDIFHQTWANYFVKFLDAYAKYGLRFWAVTAENEPTAGLFTGYPFQCLGFTPEHQRDFISRDLGPALANSSHDVKLLMLDDQRLLLPRWAEVVLSDPEAAKYVHGIAVHWYMDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVTGWTDWNLALNPEGGPNWVRNFVDSPIIVDIPKDAFYKQPMFYHLGHFSKFIPEGSQRVALVASESTDLETVALLRPDGSAVVVVLNRSSEDVPLTISDPDLGFLETVSPGYSIHTYLWRRQ

Approximately 57.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.