MMP-14 蛋白, Human (His-SUMO)

MMP-14 protein is an endopeptidase that degrades collagen and activates progelatinase A. MMP-14 Protein, Human (His-SUMO) is the recombinant human-derived MMP-14 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of MMP-14 Protein, Human (His-SUMO) is 471 a.a., with molecular weight of ~69.9 KDa.

MMP-14 蛋白是一种关键的内肽酶，靶向细胞外基质的各种成分，尤其是胶原蛋白，并在发育过程中的细胞周胶原溶解中发挥关键作用，有助于骨骼和骨骼外结缔组织的建模。此外，MMP-14 还可激活原明胶酶 A，在细胞外基质的复杂调节中发挥关键作用。除了其基质降解功能外，MMP-14 还通过裂解 PTK7 参与肌动蛋白细胞骨架重组，这突显了其参与基质重塑以外的细胞过程。此外，MMP-14 通过激活 MMP15 作为细胞生长和迁移的正调节因子，并与 pro-MMP2 一起在纤维血管组织的形成中发挥至关重要的作用。值得注意的是，MMP-14 裂解 ADGRB1，释放 vasculostatin-40，后者通过抑制血管生成发挥抗血管生成作用。这种多方面的功能强调了 MMP-14 在协调不同细胞过程中的重要性及其作为各种生理环境中的治疗靶点的潜力。

Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is 3373.72 pmol/min/µg, as measured under the described conditions.

Human

E. coli

N-6*His;N-SUMO

P50281 (Y112-V582)

4323  [NCBI]

YAIQGLKWQHNEITFCIQNYTPKVGEYATYEAIRKAFRVWESATPLRFREVPYAYIREGHEKQADIMIFFAEGFHGDSTPFDGEGGFLAHAYFPGPNIGGDTHFDSAEPWTVRNEDLNGNDIFLVAVHELGHALGLEHSSDPSAIMAPFYQWMDTENFVLPDDDRRGIQQLYGGESGFPTKMPPQPRTTSRPSVPDKPKNPTYGPNICDGNFDTVAMLRGEMFVFKERWFWRVRNNQVMDGYPMPIGQFWRGLPASINTAYERKDGKFVFFKGDKHWVFDEASLEPGYPKHIKELGRGLPTDKIDAALFWMPNGKTYFFRGNKYYRFNEELRAVDSEYPKNIKVWEGIPESPRGSFMGSDEVFTYFYKGNKYWKFNNQKLKVEPGYPKSALRDWMGCPSGGRPDEGTEEETEVIIIEVDEEGGGAVSAAAVVLPVLLLLLVLAVGLAVFFFRRHGTPRRLLYCQRSLLDKV

Approximately 69.9 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.