NAALADL1 蛋白, Human (HEK293, His)

NAALADL1 is an aminopeptidase with broad substrate specificity and shows lower activity towards substrates containing Asp or Glu at the P2' position or Pro at the P3' position. It is worth noting that it lacks activity against substrates with Pro at the P3' position and Asp or Glu at the P2' position. NAALADL1 Protein, Human (HEK293, His) is the recombinant human-derived NAALADL1 protein, expressed by HEK293 , with N-His labeled tag. The total length of NAALADL1 Protein, Human (HEK293, His) is 712 a.a., with molecular weight of ~90 kDa.

NAALADL1 是一种氨肽酶，表现出广泛的底物特异性，尽管它对 P2' 位置含有 Asp 或 Glu 或 P3' 位置含有 Pro 的底物表现出较低的活性。值得注意的是，它对 P3' 位置上有 Pro 且 P2' 位置上有 Asp 或 Glu 的底物缺乏活性。此外，NAALADL1不具有羧肽酶活性并且缺乏二肽基肽酶IV型活性。这些酶学特征强调了 NAALADL1 的微妙底物偏好和局限性，揭示了其作为具有特定底物相互作用和催化特性的氨肽酶的作用。

Measured by its binding ability in a functional ELISA. Immobilized Human NAALADL1, at 2 μg/mL (100μL/well) can bind Anti-NAALADL1 antibody. The ED₅₀ for this effect is 0.1597 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human NAALADL1, at 2μg/mL (100μL/well) can bind Anti-NAALADL1 antibody. The ED₅₀ for this effect is 0.1597μg/mL.

Human

HEK293

N-His

Q9UQQ1-1 (P29-L740)

10004  [NCBI]

PKKANSLAPQDLDLEILETVMGQLDAHRIRENLRELSREPHLASSPRDEDLVQLLLQRWKDPESGLDSAEASTYEVLLSFPSQEQPNVVDIVGPTGGIIHSCHRTEENVTGEQGGPDVVQPYAAYAPSGTPQGLLVYANRGAEEDFKELQTQGIKLEGTIALTRYGGVGRGAKAVNAAKHGVAGVLVYTDPADINDGLSSPDETFPNSWYLPPSGVERGSYYEYFGDPLTPYLPAVPSSFRVDLANVSGFPPIPTQPIGFQDARDLLCNLNGTLAPATWQGALGCHYRLGPGFRPDGDFPADSQVNVSVYNRLELRNSSNVLGIIRGAVEPDRYVLYGNHRDSWVHGAVDPSSGTAVLLELSRVLGTLLKKGTWRPRRSIVFASWGAEEFGLIGSTEFTEEFFNKLQERTVAYINVDISVFANATLRVQGTPPVQSVVFSATKEIRSPGPGDLSIYDNWIRYFNRSSPVYGLVPSLGSLGAGSDYAPFVHFLGISSMDIAYTYDRSKTSARIYPTYHTAFDTFDYVDKFLDPGFSSHQAVARTAGSVILRLSDSFFLPLKVSDYSETLRSFLQAAQQDLGALLEQHSISLGPLVTAVEKFEAEAAALGQRISTLQKGSPDPLQVRMLNDQLMLLERTFLNPRAFPEERYYSHVLWAPRTGSVVTFPGLSNACSRARDTASGSEAWAEVQRQLSIVVTALEGAAATLRPVADL

Approximately 95 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.