Neurotrophin-4 蛋白, Human

NT-4 Protein, Human is a member of neurotrophins family, binding with two distinct receptors: TrkB, high affinity receptor and p75 low-affinity neurotrophin receptor (p75NTR).

Neurotropin-4 (NT-4) 是经过充分研究的神经营养蛋白家族的一员，它通过参与神经元过程的生长、突触发育和可塑性、神经元存活、分化以及髓鞘形成来调节神经元网络的发育。Neurotropin-4 与两种不同的受体结合：TrkB、高亲和力受体和 p75 低亲和力神经营养素受体 (p75NTR)^[1]。

1. The ED₅₀ is <5 μg/mL as measured by C6 cells, corresponding to a specific activity of >2.0 × 10² units/mg.
2. Measured by its binding ability in a functional ELISA. When Recombinant Human Neurotrophin-4 is present at 5 μg/mL, can bind Recombinant Mouse TrkB. The ED₅₀ for this effect is 8.676 ng/mL.

Human

E. coli

Tag Free

P34130 (G81-A210)

4909  [NCBI]

MGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA

Approximately 17 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 50 mM acetic acid or PBS, pH 7.4.

<0.3 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O or 50 mM acetic acid. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. D'Angelo L, et al. Neurotrophin-4 in the brain of adult Nothobranchius furzeri. Ann Anat. 2016 Sep;207:47-54.  [Content Brief]