2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. O-acyltransferase 蛋白, Zea mays (Cell-Free, His)

O-acyltransferase 蛋白, Zea mays (Cell-Free, His)

目录号: HY-P702392
产品使用指南

O-酰基转移酶蛋白在甘油脂代谢中发挥着至关重要的作用，特别是在三酰基甘油的合成中。这些酶对于脂质代谢至关重要，使细胞能够转化和合成重要的脂质分子。O-acyltransferase 蛋白, Zea mays (Cell-Free, His) 是重组的 O-酰基转移酶蛋白，由 E. coli Cell-free 表达，带有 N-10*His 标签。O-acyltransferase 蛋白, Zea mays (Cell-Free, His) 全长 494 个氨基酸，分子量为 57.8 kDa。

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描述

O-acyltransferase Proteins play a crucial role in glycerolipid metabolism, particularly in the synthesis of triacylglycerol. These enzymes are essential for lipid metabolism, enabling the cell to convert and synthesize important lipid molecules. O-acyltransferase Protein, Zea mays (Cell-Free, His) is the recombinant O-acyltransferase protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of O-acyltransferase Protein, Zea mays (Cell-Free, His) is 494 a.a., with molecular weight of 57.8 kDa.
研究背景

O-酰基转移酶是甘油脂代谢的关键参与者，特别参与三酰甘油的生物合成。这种酶在脂质代谢中发挥着关键作用，促进细胞内重要脂质分子的转化和合成。
种属

Others
表达系统

E. coli Cell-free
标签

N-10*His
蛋白编号

B0LF77 (M1-R494)
基因 ID

103629820
蛋白结构
N-term
10*His
O-acyltransferase (M1-R494)
Accession # B0LF77
C-term
同用名
O-acyltransferase
氨基酸序列

MAPPPSMPAASDRAGPGRDAGDSSSLRLRRAPSADAGDLAGDSSGGLRENGEPQSPTNPPPQEQQQHEMLYYRASAPAHRRVKESPLSSDAIFRQSHAGLLNLCIVVLIAVNSRLIIENLMKYGLLIRAGFWFSARSLGDWPLLMCCLTLPVFPLVALMAEKLITRKLIGEHVVILLHIIITTSAIVYPVVVTLKCDSAVLSGFVLMFLASIMWMKLVSYAHTNYDIRVLSKSTEKGAAYGNYVDPENMKDPTFKSLVYFMLAPTLCYQPTYPQTTCIRKGWVTQQLIKCVVFTGLMGFIIEQYINPIVKNSKHPLKGNFLNAIERVLKLSVPTLYVWLCMFYCFFHLWLNIVAELLCFGDREFYKDWWNAKTVEEYWRMWNMPVHKWIIRHIYFPCIRKGFSRGVAILISFLVSAVFHEICIAVPCHIFKFWAFSGIMFQIPLVFLTRYLHATFKHVMVGNMIFWFFFSIVGQPMCVLLYYHDVMNRQAQASR

分子量

57.8 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

O-acyltransferase Protein, Zea mays (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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