OPRM1 蛋白, Human (Cell-Free, His)

The OPRM1 protein serves as a receptor for endogenous and synthetic opioids and undergoes conformational changes upon agonist binding, activating downstream signaling pathways. This includes coupling to G proteins, thereby regulating adenylyl cyclase, calcium channels, potassium channels, and intracellular signaling pathways. OPRM1 Protein, Human (Cell-Free, His) is the recombinant human-derived OPRM1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of OPRM1 Protein, Human (Cell-Free, His) is 400 a.a., with molecular weight of 47.6 kDa.

OPRM1 作为一系列内源性阿片类药物（包括 β-内啡肽和内吗啡）的受体，并对各种天然和合成阿片类药物（如吗啡、海洛因、DAMGO、芬太尼、埃托啡、丁丙诺啡和美沙酮）做出反应。激动剂结合后，受体会发生构象变化，导致下游信号级联的激活。激动剂诱导的激活涉及与非活性 GDP 结合的异三聚体 G 蛋白复合物偶联，引发 G 蛋白复合物解离，随后激活 G 蛋白 α 亚基，特别是百日咳毒素敏感的 G(i) 和 G( o) G α 蛋白。这种激活会导致多种细胞反应，包括抑制腺苷酸环化酶活性、调节钙通道、激活钾通道以及调节各种细胞内信号传导途径，例如 MAPK、PLC、PKC、PI3K 和 NF-κ。B.此外，OPRM1 会被 GPRK 亚家族蛋白激酶磷酸化，其与 β-抑制蛋白的结合对于短期受体脱敏、通过内吞作用内化以及随后的回收至关重要。该受体作为 A 类 G 蛋白偶联受体 (GPCR)，表现出与 G 蛋白的选择性时间偶联并经历快速回收，而其下调途径随激动剂而变化，并且可以依赖或独立于 G 蛋白而发生耦合。此外，OPRM1 具有与其他 GPCR 发生异源寡聚化的潜力，影响激动剂结合、信号传导和运输特性，最终在兴奋作用中发挥作用。

Human

E. coli Cell-free

N-10*His

P35372 (M1-P400)

4988

MDSSAAPTNASNCTDALAYSSCSPAPSPGSWVNLSHLDGNLSDPCGPNRTDLGGRDSLCPPTGSPSMITAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIINVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALVTIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSNIEQQNSTRIRQNTRDHPSTANTVDRTNHQLENLEAETAPLP

Monomer: 43 kDa Dimer: 100 kDa It is speculated that the protein forms a dimeric structure.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% Brij-78, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.