1. 重组蛋白
  2. Receptor Proteins
  3. P2RY12 蛋白, Human (Cell-Free, His)

P2RY12 蛋白, Human (Cell-Free, His)

目录号: HY-P702410
产品使用指南

P2RY12 蛋白是二磷酸腺苷 (ADP) 和三磷酸腺苷 (ATP) 的受体,通过 G 蛋白抑制腺苷酸环化酶第二信使系统。与尿苷二磷酸 (UDP) 和尿苷三磷酸 (UTP) 不同,P2RY12 至关重要地调节血小板聚集和凝血,是这些生理过程的关键调节剂。P2RY12 蛋白, Human (Cell-Free, His) 是重组的 P2RY12 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。P2RY12 蛋白, Human (Cell-Free, His) 全长 342 个氨基酸,分子量为 42.2 kDa。

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描述

P2RY12 Protein, a receptor for adenosine diphosphate (ADP) and adenosine triphosphate (ATP), inhibits the adenylyl cyclase second messenger system via G-proteins. Unlike uridine diphosphate (UDP) and uridine triphosphate (UTP), P2RY12 crucially regulates platelet aggregation and blood coagulation, serving as a key regulator in these physiological processes. P2RY12 Protein, Human (Cell-Free, His) is the recombinant human-derived P2RY12 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of P2RY12 Protein, Human (Cell-Free, His) is 342 a.a., with molecular weight of 42.2 kDa.

研究背景

P2RY12 蛋白作为二磷酸腺苷 (ADP) 和三磷酸腺苷 (ATP) 的受体,与抑制腺苷酸环化酶第二信使系统的 G 蛋白偶联。该受体不被尿苷二磷酸 (UDP) 和尿苷三磷酸 (UTP) 激活。P2RY12 在正常血小板聚集和凝血过程中发挥着至关重要的作用,是调节这些基本生理功能的关键组成部分。

种属

Human

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

Q9H244 (M1-M342)

基因 ID

64805

蛋白结构
N-term
10*His
P2RY12 (M1-M342)
Accession # Q9H244
C-term
同用名
P2Y purinoceptor 12; ADP-glucose receptor; ADPG-R; P2T(AC); P2Y(AC); P2Y(cyc); P2Y12 platelet ADP receptor; P2Y(ADP); SP1999
氨基酸序列

MQAVDNLTSAPGNTSLCTRDYKITQVLFPLLYTVLFFVGLITNGLAMRIFFQIRSKSNFIIFLKNTVISDLLMILTFPFKILSDAKLGTGPLRTFVCQVTSVIFYFTMYISISFLGLITIDRYQKTTRPFKTSNPKNLLGAKILSVVIWAFMFLLSLPNMILTNRQPRDKNVKKCSFLKSEFGLVWHEIVNYICQVIFWINFLIVIVCYTLITKELYRSYVRTRGVGKVPRKKVNVKVFIIIAVFFICFVPFHFARIPYTLSQTRDVFDCTAENTLFYVKESTLWLTSLNACLDPFIYFFLCKSFRNSLISMLKCPNSATSLSQDNRKKEQDGGDPNEETPM

分子量

42.2 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

P2RY12 Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
P2RY12 Protein, Human (Cell-Free, His)
目录号:
HY-P702410
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