p38 gamma/MAPK12 蛋白, Human (sf9)

p38 gamma/MAPK12 Protein, a vital serine/threonine kinase in the MAP kinase pathway, responds to extracellular stimuli like pro-inflammatory cytokines or stress. Leading to the activation of transcription factors such as ELK1 and ATF2, p38 MAPKs phosphorylate around 200 to 300 substrates each. MAPK12 contributes to myoblast differentiation, down-regulates cyclin D1 in response to hypoxia, and inhibits cell proliferation while promoting differentiation. It phosphorylates DLG1, impacting mRNA processing during osmotic shock, regulates UV-induced checkpoint signaling, influences glucose uptake, ensures normal kinetochore localization of PLK1, and positively regulates myogenic precursor cell expansion in muscle growth and regeneration. p38 gamma/MAPK12 Protein, Human (sf9) is the recombinant human-derived p38 gamma/MAPK12 protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes) mutation. The total length of p38 gamma/MAPK12 Protein, Human (sf9) is 367 a.a., with molecular weight of ~43 kDa.

p38 gamma/MAPK12 是一种丝氨酸/苏氨酸激酶，也是 MAP 激酶信号转导途径的重要组成部分，是四种 p38 MAPK 之一，在促炎细胞因子或物理应激等细胞外刺激触发的细胞反应中发挥着关键作用。该途径导致 ELK1 和 ATF2 等转录因子的直接激活，p38 MAPK 磷酸化多种蛋白质，估计每种蛋白质大约有 200 至 300 个底物。 MAPKAPK2 是其下游激酶之一，通过磷酸化激活以进一步磷酸化其他靶标。 MAPK12 有助于成肌细胞分化，并在肾上腺细胞缺氧时下调细胞周期蛋白 D1，表明其在抑制细胞增殖、促进分化方面的作用。值得注意的是，MAPK12 磷酸化 DLG1，渗透压休克后，它会增加与核 DLG1 的关联，影响 mRNA 加工和/或基因转录，以帮助细胞适应渗透压变化。此外，MAPK12 调节紫外线诱导的检查点信号传导和修复紫外线诱导的 DNA 损伤，影响肌肉细胞中的葡萄糖摄取，并且对于 PLK1 的正常动粒定位至关重要，防止染色体不稳定并支持有丝分裂细胞的活力。其信号传导在肌肉生长和再生过程中积极调节瞬时放大生肌前体细胞的扩张。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

Tag Free

P53778 (N-G&P, M1-L367)

6300  [NCBI]

Approximately 43 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.