p53 蛋白, Rat (His)

The p53 protein acts as a tumor suppressor, regulating the cell cycle and inducing growth arrest or apoptosis. It activates genes that inhibit cell division and triggers apoptosis by controlling the expression of various proteins. It interacts with different proteins to facilitate pro-apoptotic function, but its activity can be inhibited by other proteins. Additionally, p53 is involved in oxidative stress-induced necrosis and prevents CDK7 kinase activity in response to DNA damage. It also induces the transcription of specific non-coding RNAs and regulates the circadian clock. p53 Protein, Rat (His) is the recombinant rat-derived p53 protein, expressed by E. coli, with N-6*His labeled tag. The total length of p53 Protein, Rat (His) is 391 a.a., with molecular weight of ~50 kDa.

p53 蛋白在各种肿瘤类型中充当肿瘤抑制因子，并且可以根据具体情况和细胞类型诱导生长停滞或细胞凋亡。它作为反式激活剂，通过调节一组对该过程至关重要的基因来负向控制细胞分裂，从而在调节细胞周期中发挥至关重要的作用。p53 激活的基因之一是细胞周期蛋白依赖性激酶的抑制剂。细胞凋亡可以通过刺激 BAX 和 FAS 抗原表达或抑制 Bcl-2 表达来触发。其促凋亡功能通过与 PPP1R13B/ASPP1 或 TP53BP2/ASPP2 的相互作用得到促进。然而，当 PPP1R13L/iASPP 取代与 PPP1R13B/ASPP1 或 TP53BP2/ASPP2 的相互作用时，该活性受到抑制。此外，p53 与线粒体 PPIF 合作，参与激活氧化应激诱导的坏死，这在很大程度上独立于转录。为了应对 DNA 损伤，p53 通过与 CAK 复合物结合来阻止 CDK7 激酶活性，从而阻止细胞周期进程。此外，p53 诱导长基因间非编码 RNA p21 (lincRNA-p21) 和 lincRNA-Mkln1 的转录，它们参与 TP53 依赖性转录抑制，导致细胞凋亡，并可能影响细胞周期调节。此外，p53 通过抑制 CLOCK-ARNTL/BMAL1 介导的 PER2 转录激活来调节生物钟。

Rat

E. coli

N-6*His

P10361 (1M-391D)

24842  [NCBI]

MEDSQSDMSIELPLSQETFSCLWKLLPPDDILPTTATGSPNSMEDLFLPQDVAELLEGPEEALQVSAPAAQEPGTEAPAPVAPASATPWPLSSSVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSISLNKLFCQLAKTCPVQLWVTSTPPPGTRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNPYAEYLDDRQTFRHSVVVPYEPPEVGSDYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEEHCPELPPGSAKRALPTSTSSSPQQKKKPLDGEYFTLKIRGRERFEMFRELNEALELKDARAAEESGDSRAHSSYPKTKKGQSTSRHKKPMIKKVGPDSD

Approximately 50 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.