1. 重组蛋白
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. PAK5 蛋白, Human (Baculovirus, His)

PAK5 蛋白, Human (Baculovirus, His)

目录号: HY-P72065
产品使用指南

PAK5 是一种动态丝氨酸/苏氨酸激酶,通过多种途径影响细胞骨架调节、细胞迁移、增殖和存活。激活会触发自身磷酸化,影响 RAF1 激酶活性并通过 BAD 磷酸化促进细胞存活。PAK5 蛋白, Human (Baculovirus, His) 是重组的 PAK5 蛋白,由 Sf9 insect cells 表达,带有 N-6*His 标签。PAK5 蛋白, Human (Baculovirus, His) 全长 293 个氨基酸,分子量约为 ~34.9 kDa。

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描述

PAK5 is a dynamic serine/threonine kinase that affects cytoskeletal regulation, cell migration, proliferation, and survival through multiple pathways. Activation triggers autophosphorylation, affecting RAF1 kinase activity and promoting cell survival through BAD phosphorylation. PAK5 Protein, Human (Baculovirus, His) is the recombinant human-derived PAK5 protein, expressed by Sf9 insect cells , with N-6*His labeled tag. The total length of PAK5 Protein, Human (Baculovirus, His) is 293 a.a., with molecular weight of ~34.9 kDa.

研究背景

PAK5 是一种丝氨酸/苏氨酸蛋白激酶,在多种信号传导途径中发挥着多功能作用,影响细胞骨架调节、细胞迁移、增殖和细胞存活。多种效应子(包括生长因子受体或活性 CDC42 和 RAC1)的激活可诱导构象变化以及随后多个丝氨酸和/或苏氨酸残基的自磷酸化。PAK5 的影响延伸至原癌基因 RAF1 的磷酸化,增强其激酶活性,并通过细胞死亡的 BCL2 拮抗剂 BAD 的磷酸化促进细胞存活。此外,PAK5 磷酸化 CTNND1,可能调节细胞骨架组织和细胞形态。它通过抑制 MARK2 发挥微管稳定性作用,同时破坏 F-肌动蛋白网络的稳定性,导致应力纤维和粘着斑消失。PAK5 成为多种细胞过程交叉点的关键调节因子。

种属

Human

表达系统

Sf9 insect cells

标签

N-6*His

蛋白编号

Q8TB93 (M1-Q293)

基因 ID
蛋白结构
N-term
6*His
PAK5 (M1-Q293)
Accession # Q8TB93
C-term
同用名
erine/threonine-protein kinase PAK 5; PAK5
氨基酸序列

MFGKKKKKIEISGPSNFEHRVHTGFDAQEQKFTGLPQQWHSLLADTANRPKPMVDPSCITPIQLAPMKTIVRGNKPCKETSINGLLEDFDNISVTRSNSLRKESPPTPDQGASSHGPGHAEENGFITFSQYSSESDTTADYTTEKYREKSLYGDDLDPYYRGSHAAKQNGHVMKMKHGEAYYSEVKPLKSDFARFSADYHSHLDSLSKPSEYSDLKWEYQRASSSSPLDYSFQFTPSRTAGTSGCSKESLAYSESEWGPSLDDYDRRPKSSYLNQTSPQPTMRQRSRSGSGLQ

分子量

Approximately 34.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PAK5 Protein, Human (Baculovirus, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PAK5 Protein, Human (Baculovirus, His)
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HY-P72065
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