PDGF-AA 蛋白, Human

PDGF-A protein is a key growth factor that regulates embryonic development, cell proliferation, migration, survival, and chemotaxis, and exerts potent mitogenic effects on mesenchymal cells. It is essential for alveolar septal formation, gastrointestinal tract development, interstitial cell maturation, spermatogenesis, and oligodendrocyte development, and contributes to spinal cord and cerebellar myelination. PDGF-AA Protein, Human is the recombinant human-derived PDGF-AA protein, expressed by E. coli , with tag free. The total length of PDGF-AA Protein, Human is 125 a.a., with molecular weight of ~16.0 kDa.

PDGF-A 蛋白作为调节胚胎发育、细胞增殖、迁移、存活和趋化性的关键生长因子占据中心地位。PDGF-A 因其对间充质细胞的有效促有丝分裂作用而闻名，对于各种发育过程都是不可或缺的，包括肺泡间隔的形成、胃肠道发育、Leydig 细胞成熟、精子发生和少突胶质细胞的正常发育，有助于少突胶质细胞的髓鞘形成。脊髓和小脑。此外，PDGF-A 在伤口愈合动力学中发挥着至关重要的作用。其信号通路的复杂性涉及通过与 PDGFB 形成异二聚体进行调节，这证明了其调节的多功能性。在结构上，PDGF-A采用具有反向平行二硫键连接二聚体的同二聚体构型，并与PDGFB形成异二聚体，与PDGFRA同二聚体以及PDGFRA和PDGFRB形成的异二聚体相互作用。此外，PDGF-A 表现出与 CSPG4 的相互作用，为其多方面参与细胞过程增添了另一层。

Measured  in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The  ED₅₀ for this effect is 3.691 ng/mL, corresponding to a specific  activity is 2.701×10⁵ units/mg.

Human

E. coli

Tag Free

P04085 (S87-T211)

5154  [NCBI]

SIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT

Approximately 16.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Glycine-HCl, 6% Sucrose, 4% Mannitol, 0.02% Tween 80, pH 3.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.