PHOSPHO1 蛋白, Human (His)

PHOSPHO1 Protein, a phosphatase, is crucial in generating inorganic phosphate for bone mineralization, displaying high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). It collaborates with PHOSPHO1 in skeletal mineralization, initiating hydroxyapatite crystallization in matrix vesicles (MVs), while ALPL/TNAP catalyzes hydroxyapatite crystallization in the extracellular matrix. PHOSPHO1 Protein, Human (His) is the recombinant human-derived PHOSPHO1 protein, expressed by E. coli , with N-His labeled tag. The total length of PHOSPHO1 Protein, Human (His) is 267 a.a., with molecular weight of ~32 kDa.

PHOSPHO1 蛋白是一种对磷酸乙醇胺 (PEA) 和磷酸胆碱 (PCho) 具有高活性的磷酸酶，在骨矿化无机磷酸盐的生成中发挥着至关重要的作用。它在骨骼矿化中与 PHOSPHO1 协同作用，其中 PHOSPHO1 启动基质囊泡 (MV) 中的羟基磷灰石结晶，而 ALPL/TNAP 催化羟基磷灰石结晶在细胞外基质中的扩散。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

Q8TCT1 (M1-C267)

162466  [NCBI]

MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDENSDDSIVRAAPGQRLPESLRATYREGFYNEYMQRVFKYLGEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEVILISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGLLALRPFHTHSCARCPANMCKHKVLSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEPSSFRASVVPWETAADVRLHLQQVLKSC

Approximately 32 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 300mM L-Arginine, pH 7.0 or 20 mM PB, 200 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.