1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PIP4K2C 蛋白, Human

PIP4K2C 蛋白, Human

目录号: HY-P701750
产品使用指南

PIP4K2C蛋白是一种低活性磷脂酰肌醇5-磷酸4-激酶,充当GTP传感器,其GTP依赖性激酶活性高于ATP依赖性活性。除了其催化作用外,PIP4K2C 通过与 PIP5K 相互作用独立负调节胰岛素信号传导,并抑制 PIP5K 介导的 PtdIns(4,5)P2 合成。PIP4K2C 蛋白, Human 是重组的 PIP4K2C 蛋白,由 E. coli 表达,不带标签。PIP4K2C 蛋白, Human 全长 420 个氨基酸。

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规格 是否有货
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100 μg   询价  

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描述

PIP4K2C protein is a low-activity phosphatidylinositol 5-phosphate 4-kinase that functions as a GTP sensor, and its GTP-dependent kinase activity is higher than ATP-dependent activity. In addition to its catalytic role, PIP4K2C independently negatively regulates insulin signaling by interacting with PIP5K and inhibits PIP5K-mediated PtdIns(4,5)P2 synthesis. PIP4K2C Protein, Human is the recombinant human-derived PIP4K2C protein, expressed by E. coli , with tag free. The total length of PIP4K2C Protein, Human is 420 a.a., .

研究背景

PIP4K2C 蛋白是一种磷脂酰肌醇 5-磷酸 4-激酶,表现出低酶活性,被提议用作 GTP 传感器,与 ATP 依赖性激酶活性相比,显示出更高的 GTP 依赖性激酶活性。除了其催化作用之外,PIP4K2C 与其他 PIP4K 一样,通过催化独立机制在负调节胰岛素信号传导中发挥着重要作用。这涉及与 PIP5K 的相互作用,导致 PIP5K 介导的磷脂酰肌醇 4,5-二磷酸 (PtdIns(4,5)P2) 合成受到抑制,并抑制胰岛素依赖性转化为磷脂酰肌醇 3,4,5-三磷酸 (PtdIns( 3,4,5)P3)。这些调节作用强调了 PIP4K2C 在与胰岛素信号传导和脂质代谢相关的细胞过程中的复杂参与。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q8TBX8 (A2-A421)

基因 ID

79837

蛋白结构
N-term
PIP4K2C (A2-A421)
Accession # Q8TBX8
C-term
同用名
PIP4K2C; Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma; Phosphatidylinositol 5-phosphate 4-kinase type II gamma; PI(5)P 4-kinase type II gamma; PIP4KII-gamma
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PIP4K2C Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PIP4K2C Protein, Human
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HY-P701750
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