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  3. PITPNA 蛋白, Human (His)

PITPNA 蛋白, Human (His)

目录号: HY-P71207
产品使用指南

PITPNA 蛋白催化磷脂酰肌醇 (PI) 和磷脂酰胆碱 (PC) 在膜之间的转移,在 sn-1 和 sn-2 位置显示出对较短饱和或单饱和酰基链的偏好。对于 PC,优先顺序为 C16:1 > C16:0 > C18:1 > C18:0 > C20:4,对于 PI,优先顺序为 C16:1 > C16:0 > C18:1 > C18:0 > C20 :4 > C20:3。PITPNA 蛋白, Human (His) 是重组的 PITPNA 蛋白,由 E. coli 表达,带有 N-6*His 标签。PITPNA 蛋白, Human (His) 全长 270 个氨基酸,分子量分别为 16 & 22 & 38 kDa。

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描述

PITPNA Protein catalyzes phosphatidylinositol (PI) and phosphatidylcholine (PC) transfer between membranes, displaying a preference for shorter saturated or monosaturated acyl chains at sn-1 and sn-2 positions. For PC, the preference order is C16:1 > C16:0 > C18:1 > C18:0 > C20:4, and for PI, it is C16:1 > C16:0 > C18:1 > C18:0 > C20:4 > C20:3. PITPNA Protein, Human (His) is the recombinant human-derived PITPNA protein, expressed by E. coli , with N-6*His labeled tag. The total length of PITPNA Protein, Human (His) is 270 a.a., with molecular weight of 16 & 22 & 38 kDa, respectively.

研究背景

PITPNA 蛋白充当磷脂酰肌醇 (PI) 和磷脂酰胆碱 (PC) 在细胞膜之间转移的催化剂。该酶对 sn-1 和 sn-2 位点具有较短饱和或单饱和酰基链的 PI 和 PC 分子表现出显着的偏好。PC基材的优先顺序为C16:1 > C16:0 > C18:1 > C18:0 > C20:4,PI基材的优先顺序为C16:1 > C16:0 > C18:1 > C18:0 > C20:4 > C20:3。PITPNA 介导的动态脂质转移有助于调节细胞膜中的脂质组成,影响各种细胞过程并维持膜稳态。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q00169 (M1-D270)

基因 ID
蛋白结构
N-term
6*His
PITPNA (M1-D270)
Accession # Q00169
C-term
同用名
Phosphatidylinositol Transfer Protein Alpha Isoform; PI-TP-Alpha; PtdIns Transfer Protein Alpha; PtdInsTP Alpha; PITPNA; PITPN
氨基酸序列

MVLLKEYRVILPVSVDEYQVGQLYSVAEASKNETGGGEGVEVLVNEPYEKDGEKGQYTHKIYHLQSKVPTFVRMLAPEGALNIHEKAWNAYPYCRTVITNEYMKEDFLIKIETWHKPDLGTQENVHKLEPEAWKHVEAVYIDIADRSQVLSKDYKAEEDPAKFKSIKTGRGPLGPNWKQELVNQKDCPYMCAYKLVTVKFKWWGLQNKVENFIHKQERRLFTNFHRQLFCWLDKWVDLTMDDIRRMEEETKRQLDEMRQKDPVKGMTADD

分子量

16&22&38 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PITPNA Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PITPNA Protein, Human (His)
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