1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PK3C3 蛋白, Human (Sf9, His, GST)

PK3C3 蛋白, Human (Sf9, His, GST)

目录号: HY-P701752
产品使用指南

PI3KC3 蛋白是 PI3K 复合物的催化亚基,介导磷脂酰肌醇 3-磷酸的形成。PI3KC3-C1 启动自噬体,而 PI3KC3-C2 促进自噬体成熟和内吞作用。PK3C3 蛋白, Human (Sf9, His, GST) 是重组的 PK3C3 蛋白,由 Sf9 insect cells 表达,带有 N-8*His, N-GST 标签。PK3C3 蛋白, Human (Sf9, His, GST) 全长 886 个氨基酸。

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描述

The PI3KC3 protein is the catalytic subunit of the PI3K complex and mediates the formation of phosphatidylinositol 3-phosphate. PI3KC3-C1 initiates autophagosomes, whereas PI3KC3-C2 promotes autophagosome maturation and endocytosis. PK3C3 Protein, Human (Sf9, His, GST) is the recombinant human-derived PK3C3 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of PK3C3 Protein, Human (Sf9, His, GST) is 886 a.a., .

研究背景

PK3C3 充当 PI3K 复合物的催化亚基,协调磷脂酰肌醇 3-磷酸的形成。PK3C3 的各种复杂形式在不同的膜运输途径中发挥着关键作用,其中 PI3KC3-C1 启动自噬体,PI3KC3-C2 促进自噬体的成熟和内吞作用。在 PI3KC3-C1 中,PK3C3 在囊泡出芽前积极促进内质网膜曲率的形成。此外,PK3C3 参与降解性内吞运输的调节,这对于胞质分裂中的脱离步骤至关重要,可能在 PI3KC3-C2 的背景下。此外,PK3C3 在溶酶体酶前体转运至溶酶体中发挥着至关重要的作用,并且是从早期内体到晚期内体的进展所必需的。值得注意的是,PK3C3 的激酶活性对于微生物感染期间 SARS 冠状病毒 (SARS-CoV-2) 的复制至关重要。

种属

Human

表达系统

Sf9 insect cells

标签

N-8*His;N-GST

蛋白编号

Q8NEB9 (G2-K887)

基因 ID

5289

蛋白结构
N-term
8*His-GST
PK3C3 (G2-K887)
Accession # Q8NEB9
C-term
同用名
PIK3C3; Phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PK3C3 Protein, Human (Sf9, His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PK3C3 Protein, Human (Sf9, His, GST)
目录号:
HY-P701752
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