1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PKC iota 蛋白, Human (sf9, GST)

PKC iota 蛋白, Human (sf9, GST)

目录号: HY-P74630
产品使用指南

PKC iota 蛋白是钙依赖性细胞粘附蛋白家族的成员,在细胞相互作用中发挥着关键作用。PKC iota 通过钙依赖性机制发挥作用,并表现出对同质相互作用、连接细胞的偏好。PKC iota 蛋白, Human (sf9, GST) 是重组的 PKC iota 蛋白,由 Sf9 insect cells 表达,带有 N-GST 标签。PKC iota 蛋白, Human (sf9, GST) 全长 587 个氨基酸,分子量约为 ~100 kDa。

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描述

PKC iota proteins are members of the calcium-dependent cell adhesion protein family and play critical roles in cellular interactions. PKC iota acts through a calcium-dependent mechanism and exhibits a preference for homogeneous interacting, connecting cells. PKC iota Protein, Human (sf9, GST) is the recombinant human-derived PKC iota protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of PKC iota Protein, Human (sf9, GST) is 587 a.a., with molecular weight of ~100 kDa.

研究背景

Cadherin-17 属于钙依赖性细胞粘附蛋白家族,是细胞相互作用的关键成分。CDH17 等钙粘蛋白通过钙依赖性机制发挥作用,表现出亲同性相互作用的倾向,在连接细胞时优先与自身相互作用。这种独特的特性使钙粘蛋白可能有助于异质细胞类型的复杂分选过程。具体而言,LI-钙粘蛋白(该家族中的一种亚型)可能在肝脏和肠道等重要器官的形态组织中发挥至关重要的作用。这凸显了钙粘蛋白(包括 CDH17)在维持特定生理环境下的组织完整性和结构组织方面所发挥的多样化和专门功能。

种属

Human

表达系统

Sf9 insect cells

标签

N-GST

蛋白编号

P41743 (M10-V596)

基因 ID
蛋白结构
N-term
GST
PKC iota (M10-V596)
Accession # P41743
C-term
同用名
Protein kinase C iota type; PRKC-lambda/iota; nPKC-iota; PRKCI; DXS1179E
分子量

Approximately 100 kDa

纯度

Greater than 88% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PKC iota Protein, Human (sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PKC iota Protein, Human (sf9, GST)
目录号:
HY-P74630
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