2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. PKLR 蛋白, Rat (P. pastoris, His)

PKLR 蛋白, Rat (P. pastoris, His)

目录号: HY-P700578
产品使用指南

PKLR 蛋白是一种丙酮酸激酶，在糖酵解、将磷酸烯醇丙酮酸转化为丙酮酸并产生 ATP 中发挥着至关重要的作用。作为一种关键酶，PKLR 介导糖酵解的最后步骤，有助于能量产生。其催化活性影响丙酮酸，丙酮酸是一种对细胞过程有影响的中心代谢物，包括能量代谢和三羧酸 (TCA) 循环。PKLR 在糖酵解通量中的重要性强调了其在细胞能量稳态中的重要作用，为代谢途径的治疗干预提供了潜力。PKLR 蛋白, Rat (P. pastoris, His) 是重组的 PKLR 蛋白，由 P. pastoris 酵母表达，带有 N-6*His 标签。PKLR 蛋白, Rat (P. pastoris, His) 全长 574 个氨基酸，分子量为 64.2 kDa。

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描述

PKLR Protein, a pyruvate kinase, plays a crucial role in glycolysis, converting phosphoenolpyruvate to pyruvate and generating ATP. As a key enzyme, PKLR mediates the final steps of glycolysis, contributing to energy production. Its catalytic activity influences pyruvate, a central metabolite with implications for cellular processes, including energy metabolism and the tricarboxylic acid (TCA) cycle. PKLR's significance in glycolytic flux underscores its essential role in cellular energy homeostasis, presenting potential for therapeutic interventions in metabolic pathways. PKLR Protein, Rat (P. pastoris, His) is the recombinant rat-derived PKLR protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of PKLR Protein, Rat (P. pastoris, His) is 574 a.a., with molecular weight of 64.2 kDa.
研究背景

PKLR 蛋白被鉴定为丙酮酸激酶，通过催化磷酸烯醇丙酮酸转化为丙酮酸并伴随 ATP 的合成，在糖酵解中发挥关键作用。作为该代谢途径中的关键酶，PKLR 通过促进糖酵解的最后步骤，充当能量产生的关键介质。PKLR 的催化活性有助于生成丙酮酸，丙酮酸是一种对各种细胞过程有影响的中心代谢物，包括能量代谢和三羧酸 (TCA) 循环。PKLR 在糖酵解通量中的重要性强调了其在细胞能量稳态中的基本作用，并强调了其作为旨在调节代谢途径的治疗干预目标的潜力。
种属

Rat
表达系统

P. pastoris
标签

N-6*His
蛋白编号

P12928 (M1-S574)
基因 ID

24651  [NCBI]
蛋白结构
N-term
6*His
PKLR (M1-S574)
Accession # P12928
C-term
同用名
PKLR; pyruvate kinase, liver and RBC; PK1; PKL; PKR; RPK; PKRL; pyruvate kinase isozymes R/L; pyruvate kinase 1; OTTHUMP00000034069; pyruvate kinase type L; pyruvate kinase isozyme R/L; R-type/L-type pyruvate kinase; red cell/liver pyruvate kinase; pyruvate kinase, liver and blood cell; EC 2.7.1.40
氨基酸序列

MSVQENTLPQQLWPWIFRSQKDLAKSALSGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS

分子量

64.2 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

PKLR Protein, Rat (P. pastoris, His) 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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