1. 重组蛋白
  2. Enzymes & Regulators
  3. PR 蛋白, Human (His)

PR 蛋白, Human (His)

目录号: HY-P701948
产品使用指南

PR蛋白是PP2A的B调节亚基,调节底物选择性、催化活性并指导酶定位。PP2A 是一种磷酸酶复合物,由 36 kDa 催化亚基(亚基 C)和 65 kDa 恒定调节亚基(PR65 或亚基 A)组成。PR 蛋白, Human (His) 是重组的 PR 蛋白,由 E. coli 表达,带有 N-6*His 标签。PR 蛋白, Human (His) 全长 285 个氨基酸。

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描述

PR protein is the B regulatory subunit of PP2A, which regulates substrate selectivity, catalytic activity and directs enzyme localization. PP2A is a phosphatase complex consisting of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A). PR Protein, Human (His) is the recombinant human-derived PR protein, expressed by E. coli , with N-6*His labeled tag. The total length of PR Protein, Human (His) is 285 a.a., .

研究背景

PR 蛋白作为 PP2A 的 B 调节亚基,参与调节底物选择性、催化活性以及引导催化酶定位到特定的亚细胞区室。PP2A 是一种磷酸酶复合物,由具有 36 kDa 催化亚基(亚基 C)和 65 kDa 恒定调节亚基(PR65 或亚基 A)的核心酶组成。该核心二聚体与各种调节亚基相关,包括 R2/B/PR55/B55、R3/B''/PR72/PR130/PR59、R5/B'/B56 家族、48 kDa 可变调节亚基、病毒蛋白和细胞信号分子。PR 蛋白与其他调节亚基之间的相互作用强调了其在塑造 PP2A 酶的底物特异性、催化功能和亚细胞定位方面的多方面作用,从而有助于其多样化的细胞功能。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q06190 (M786-F1070)

基因 ID

5523

蛋白结构
N-term
6*His
PR (M786-F1070)
Accession # Q06190
C-term
同用名
PPP2R3A; Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha; PP2A subunit B isoform PR72/PR130; PP2A subunit B isoform R3 isoform; PP2A subunit B isoforms B''-PR72/PR130; PP2A subunit B isoforms B72/B130; Serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PR Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HY-P701948
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