1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Protease 7/OmpT 蛋白, E.coli (His)

Protease 7/OmpT 蛋白, E.coli (His)

目录号: HY-P71483
COA 产品使用指南

蛋白酶7/OmpT蛋白是一种多功能酶,可以裂解多种底物,例如T7 RNA聚合酶、铁肠杆菌素受体蛋白(FEP)和抗菌肽鱼精蛋白。其对配对碱性残基的独特特异性表明对特定氨基酸构型的偏好。Protease 7/OmpT 蛋白, E.coli (His) 是重组的 蛋白酶 7/OmpT 蛋白,由 E. coli 表达,带有 N-6*His 标签。Protease 7/OmpT 蛋白, E.coli (His) 全长 297 个氨基酸,分子量约为 ~37.5 kDa。

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
20 μg ¥2800 In-stock
50 μg ¥5450 In-stock
100 μg ¥8500 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Protease 7/OmpT protein is a multifunctional enzyme that can cleave a variety of substrates, such as T7 RNA polymerase, ferric enterobactin receptor protein (FEP), and the antimicrobial peptide protamine. Its unique specificity for paired basic residues suggests a preference for specific amino acid configurations. Protease 7/OmpT Protein, E.coli (His) is the recombinant E. coli-derived Protease 7/OmpT protein, expressed by E. coli , with N-6*His labeled tag. The total length of Protease 7/OmpT Protein, E.coli (His) is 297 a.a., with molecular weight of ~37.5 kDa.

研究背景

Protease 7/OmpT 蛋白是一种多功能酶,能够裂解各种底物,包括 T7 RNA 聚合酶、铁肠杆菌素受体蛋白 (FEP)、抗菌肽鱼精蛋白和其他蛋白质。值得注意的是,这种蛋白酶对配对碱性残基表现出明显的特异性,反映了它对特定氨基酸构型的偏好。蛋白酶 7/OmpT 靶向一系列蛋白质的能力,加上其对配对碱性残基的特异性识别,强调了其在细胞过程中的重要性,并凸显了其作为生物技术应用中分子工具的潜力。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

E.coli

表达系统

E. coli

标签

N-6*His

蛋白编号

P09169 (21S-317F)

基因 ID

945185  [NCBI]

蛋白结构
N-term
6*His
OmpT (21S-317F)
Accession # P09169
C-term
同用名
ompT; b0565; JW0554; Protease 7; EC 3.4.23.49; Omptin; Outer membrane protein 3B; Protease A; Protease VII
氨基酸序列

STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF

分子量

Approximately 37.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl,1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Protease 7/OmpT Protein, E.coli (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Protease 7/OmpT Protein, E.coli (His)
目录号:
HY-P71483
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