SHP-2 蛋白, Human (T253M, Q257L, His)

The SHP-2 protein is a key player downstream of the receptor and cytoplasmic protein tyrosine kinases, facilitating signal transduction from the cell surface to the nucleus. It actively regulates the MAPK pathway and dephosphorylates key substrates, including GAB1, ARHGAP35, EGFR, ROCK2 ("Tyr-722"), and CDC73. SHP-2 Protein, Human (T253M, Q257L, His) is the recombinant human-derived SHP-2 protein, expressed by E. coli , with N-6*His labeled tag and T253M, Q257L, , , mutation. The total length of SHP-2 Protein, Human (T253M, Q257L, His) is 592 a.a., with molecular weight of 70-75 kDa.

SHP-2 蛋白在各种受体和细胞质蛋白酪氨酸激酶的下游发挥作用，参与从细胞表面到细胞核的信号转导。它积极调节 MAPK 信号转导通路，并在关键底物去磷酸化中发挥关键作用，包括 GAB1、ARHGAP35、EGFR、ROCK2（位于“Tyr-722”）和 CDC73。此外，SHP-2 使 SOX9 的酪氨酸残基去磷酸化，导致 SOX9 失活并促进骨化。此外，它还针对酪氨酸磷酸化的 NEDD9/CAS-L 进行去磷酸化，突出了其在调节细胞信号级联中的多种作用。

Human

E. coli

N-6*His

Q06124 (T2-R593, T253M, Q257L)

5781  [NCBI]

TSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFEMLQQLECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR

70-75 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 40 mM Tris, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.