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  3. RBP4 蛋白, Cynomolgus (HEK293, His)

RBP4 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P77171
COA 产品使用指南

RBP4 蛋白是血浆中重要的视黄醇转运蛋白,介导从肝脏储存到外周组织的转运。它还通过与 TTR 相互作用增强视黄醇转运。RBP4 蛋白, Cynomolgus (HEK293, His) 是重组的 RBP4 蛋白,由 HEK293 表达,带有 C-His 标签。RBP4 蛋白, Cynomolgus (HEK293, His) 全长 183 个氨基酸,分子量约为 ~23 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

RBP4 Protein, a vital retinol transporter in blood plasma, mediates the transfer from liver stores to peripheral tissues. It also enhances retinol transport by interacting with TTR. RBP4 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived RBP4 protein, expressed by HEK293 , with C-His labeled tag. The total length of RBP4 Protein, Cynomolgus (HEK293, His) is 183 a.a., with molecular weight of ~23 kDa.

研究背景

RBP4 蛋白是一种视黄醇结合蛋白,在血浆中视黄醇的转运中发挥着至关重要的作用。它充当介质,促进视黄醇从肝脏储存转移到周围组织。此外,RBP4 与 TTR 相互作用,进一步促进其在视黄醇运输中的功能。

生物活性

Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. The 50% binding concentration (ED50) is 0.795 μM, as measured under the described conditions.

种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

A0A2K5W172 (E19-L201)

基因 ID

102136826  [NCBI]

蛋白结构
N-term
RBP4 (E19-L201)
Accession # A0A2K5W172
His
C-term
同用名
Retinol-Binding Protein 4; Plasma Retinol-Binding Protein; PRBP; RBP; RBP4
氨基酸序列

ERDCRVSSFRVKENFDKARFSGTWYAMAKKDPEGLFLQDNIVAEFSVDETGQMSATAKGRVRLLNNWDVCADMVGTFTDTEDPAKFKMKYWGVASFLQKGNDDHWIIDTDYDTYAVQYSCRLLNLDGTCADSYSFVFSRDPNGLPPEAQRIVRQRQEELCLARQYRLIVHNGYCDGRSERNLL

分子量

Approximately 23 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

RBP4 Protein, Cynomolgus (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
RBP4 Protein, Cynomolgus (HEK293, His)
目录号:
HY-P77171
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