REG-3 alpha/REG3A 蛋白, Human (HEK293, His)

REG-3 alpha/REG3A Protein, a bactericidal C-type lectin, selectively targets Gram-positive bacteria by binding to peptidoglycan carbohydrate moieties. Its antimicrobial action extends to membrane phospholipid binding, forming pores that contribute to bacterial elimination. As a hormone, REG-3 alpha responds to IL17A and gut microbiome signals. Upon secretion, it activates EXTL3 receptor, initiating cell-specific pathways. In keratinocytes, REG-3 alpha regulates proliferation and differentiation through EXTL3-PI3K-AKT after skin injury, while in the pancreas, it stimulates beta-cell proliferation. REG-3 alpha/REG3A Protein, Human (HEK293, His) is the recombinant human-derived REG-3 alpha/REG3A protein, expressed by HEK293, with C-His labeled tag. The total length of REG-3 alpha/REG3A Protein, Human (HEK293, His) is 149 a.a., with molecular weight of ~18 kDa.

REG-3 alpha/REG3A 是一种杀菌 C 型凝集素，专门针对革兰氏阳性菌，通过与肽聚糖表面暴露的碳水化合物部分结合来介导细菌杀灭。其抗菌作用延伸至结合膜磷脂，形成六聚膜渗透寡聚孔，有助于消除细菌。作为一种激素，REG-3 α 可对各种刺激做出反应，包括 IL17A 等抗炎信号和肠道微生物组。在多种细胞类型分泌后，REG-3 α 会激活其受体 EXTL3，启动细胞特异性信号传导途径。IL17A 诱导的角质形成细胞中 REG-3 α 的表达通过激活 EXTL3-PI3K-AKT 信号通路来调节皮肤损伤后角质形成细胞的增殖和分化。同时，REG-3 α 通过抑制 IL6 和 TNF 等炎症细胞因子来抑制皮肤炎症。值得注意的是，在胰腺中，REG-3 α 使 β 细胞膜透化并刺激其增殖。

Measured by its ability to enhance the outgrowth of SH-SY5Y cells. The ED₅₀ of this effect is 4.456 μg/mL, corresponding to a specific activity is 224.42 units/mg.

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  Measured by its ability to enhance the outgrowth of SH-SY5Y cells. The ED₅₀ of this effect is 4.456 μg/mL, corresponding to a specific activity is 224.42 units/mg.

Human

HEK293

C-His

Q06141-1 (E27-D175)

5068  [NCBI]

EEPQRELPSARIRCPKGSKAYGSHCYALFLSPKSWTDADLACQKRPSGNLVSVLSGAEGSFVSSLVKSIGNSYSYVWIGLHDPTQGTEPNGEGWEWSSSDVMNYFAWERNPSTISSPGHCASLSRSTAFLRWKDYNCNVRLPYVCKFTD

Approximately 18 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.