RFK 蛋白, Human (His)

RFK protein plays a key role in cellular metabolism by catalyzing the phosphorylation of riboflavin (vitamin B2) to generate flavin mononucleotide (FMN), making it the rate-limiting enzyme in FAD synthesis. This enzyme activity is critical for tumor necrosis factor (TNF)-induced reactive oxygen species (ROS) generation. RFK Protein, Human (His) is the recombinant human-derived RFK protein, expressed by E. coli , with N-His labeled tag. The total length of RFK Protein, Human (His) is 155 a.a., with molecular weight of ~18 kDa.

RFK 蛋白作为黄素腺嘌呤二核苷酸 (FAD) 合成中的限速酶，催化核黄素（维生素 B2）磷酸化形成黄素单核苷酸 (FMN)。这种酶活性对于 FAD 的产生至关重要，FAD 是参与细胞内各种氧化还原反应的关键辅助因子。除了在核黄素代谢中的作用外，RFK 还参与调节肿瘤坏死因子 (TNF) 诱导的活性氧 (ROS) 产生。通过与 TNFRSF1A 和 CYBA 的相互作用，RFK 在物理和功能上将 TNFRSF1A 与 NADPH 氧化酶（ROS 生成中的关键酶复合物）偶联。RFK 的 TNF 激活可能会增强 FAD 在 NADPH 氧化酶中的掺入，突出了 RFK 在协调这种氧化应激诱导酶复合物的组装和激活的关键步骤中的重要性。参考资料：所提供的信息基于截至 2022 年 1 月的一般知识，未引用具体参考资料。

Measured by its ability to combine with the substrate ATP and riboflavin reaction produces the ADP ability to measure. The specific activity is 3681.943 pmoL/min/μg, as measured under the described conditions.

Human

E. coli

N-His

Q969G6 (M1-H155)

55312  [NCBI]

MRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH

Approximately 18 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.