1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. RIZ1 蛋白, Human (GST)

RIZ1 蛋白, Human (GST)

目录号: HY-P76580
产品使用指南

RIZ1 蛋白是一种 S-腺苷-L-甲硫氨酸依赖性组蛋白甲基转移酶,可特异性甲基化组蛋白 H3 的“Lys-9”。此外,它还充当 DNA 结合转录因子,显示出对 HMOX1 基因内的 MTE 的亲和力。作为一种潜在的激活剂,RIZ1 表明除了组蛋白修饰之外,它还对 HMOX1 基因表达具有调节作用。RIZ1 蛋白, Human (GST) 是重组的 RIZ1 蛋白,由 E. coli 表达,带有 N-GST 标签。RIZ1 蛋白, Human (GST) 全长 200 个氨基酸,分子量约为 ~49.6 kDa。

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描述

RIZ1 Protein, an S-adenosyl-L-methionine-dependent histone methyltransferase, specifically methylates 'Lys-9' of histone H3. Additionally, it acts as a DNA-binding transcription factor, showing affinity for the MTE within the HMOX1 gene. Implicated as a potential activator, RIZ1 suggests a regulatory role in HMOX1 gene expression beyond histone modification. RIZ1 Protein, Human (GST) is the recombinant human-derived RIZ1 protein, expressed by E. coli , with N-GST labeled tag. The total length of RIZ1 Protein, Human (GST) is 200 a.a., with molecular weight of ~49.6 kDa.

研究背景

RIZ1 蛋白作为 S-腺苷-L-甲硫氨酸依赖性组蛋白甲基转移酶,表现出对组蛋白 H3 的“Lys-9”甲基化的特异性。除了其组蛋白修饰作用之外,RIZ1 还可以充当 DNA 结合转录因子,与 HMOX1(血红素加氧酶 1)基因内的巨噬细胞特异性 TPA 响应元件 (MTE) 具有亲和力。在这种情况下,它被认为是 HMOX1 的潜在转录激活剂,表明该基因的表达具有调节作用。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

Q13029 (M1-A200)

基因 ID
蛋白结构
N-term
GST
RIZ1 (M1-A200)
Accession # Q13029
C-term
同用名
PR domain zinc finger protein 2; MTB-ZF; Zinc finger protein RIZ; PRDM2; KMT8; RIZ
分子量

Approximately 49.6 kDa

纯度

Greater than 86% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

RIZ1 Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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