1. 重组蛋白
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. RNF113A 蛋白, Human (His)

RNF113A 蛋白, Human (His)

目录号: HY-P701545
产品使用指南

RNF113A 是重要的剪接体成分,参与前 mRNA 剪接并有助于小剪接体内的 U12 型内含子剪接。作为一种 E3 泛素蛋白连接酶,它催化泛素向靶蛋白的转移。RNF113A 蛋白, Human (His) 是重组的 RNF113A 蛋白,由 E. coli 表达,带有 N-6*His 标签。RNF113A 蛋白, Human (His) 全长 342 个氨基酸。

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描述

RNF113A is an important spliceosome component that participates in pre-mRNA splicing and contributes to U12-type intron splicing within the small spliceosome. As an E3 ubiquitin protein ligase, it catalyzes the transfer of ubiquitin to target proteins. RNF113A Protein, Human (His) is the recombinant human-derived RNF113A protein, expressed by E. coli , with N-6*His labeled tag. The total length of RNF113A Protein, Human (His) is 342 a.a., .

研究背景

RNF113A 作为剪接体的重要组成部分,参与前 mRNA 剪接过程,并作为次要剪接体的组成部分参与 U12 型内含子的剪接。此外,RNF113A 还充当 E3 泛素蛋白连接酶,催化泛素转移到靶蛋白上。值得注意的是,它通过非规范的“Lys-63”连接的多聚泛素链催化 SNRNP200/BRR2 的多聚泛素化。这种多面蛋白通过合成“Lys-63”连接的多聚泛素链,将 ALKBH3 和 ASCC 复合物招募到烷化剂诱导的 DNA 损伤位点,在 DNA 修复中发挥关键作用。此外,RNF113A 通过泛素化 CXCR4 来调节信号传导终止,导致其降解,从而导致 CXCR4 信号传导停止。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

O15541 (A2-T343)

基因 ID

7737

蛋白结构
N-term
6*His
RNF113A (A2-T343)
Accession # O15541
C-term
同用名
RNF113A; E3 ubiquitin-protein ligase RNF113A; Cwc24 homolog; RING finger protein 113A; Zinc finger protein 183
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
RNF113A Protein, Human (His)
目录号:
HY-P701545
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