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  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. RSK3 蛋白, Human (sf9, GST)

RSK3 蛋白, Human (sf9, GST)

目录号: HY-P76011
产品使用指南

RSK3 是 ERK 信号传导下游的激酶，对有丝分裂和应激诱导的转录因子激活至关重要。它调节翻译，影响增殖、存活和分化。在上皮性卵巢癌细胞中，RSK3 可能充当肿瘤抑制因子，突显了其在细胞稳态中的多种作用以及在癌症生物学中的潜在影响。RSK3 蛋白, Human (sf9, GST) 是重组的 RSK3 蛋白，由 Sf9 insect cells 表达，带有 N-GST 标签。RSK3 蛋白, Human (sf9, GST) 全长 733 个氨基酸，分子量约为 ~110 kDa。

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描述

RSK3, a kinase downstream of ERK signaling, crucially mediates mitogenic and stress-induced transcription factor activation. It regulates translation, impacting proliferation, survival, and differentiation. In epithelial ovarian cancer cells, RSK3 may act as a tumor suppressor, highlighting its diverse roles in cellular homeostasis and potential implications in cancer biology. RSK3 Protein, Human (sf9, GST) is the recombinant human-derived RSK3 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of RSK3 Protein, Human (sf9, GST) is 733 a.a., with molecular weight of ~110 kDa.
研究背景

RSK3 是一种丝氨酸/苏氨酸蛋白激酶，在 ERK（MAPK1/ERK2 和 MAPK3/ERK1）信号传导下游发挥作用，在介导有丝分裂和应激诱导的转录因子激活中发挥着至关重要的作用。它的参与延伸到翻译的调节和细胞过程的介导，例如增殖、存活和分化。值得注意的是，在上皮性卵巢癌细胞中，RSK3 可能充当肿瘤抑制因子，进一步强调了其在细胞稳态中的多种作用以及在癌症生物学中的潜在影响。
种属

Human
表达系统

Sf9 insect cells
标签

N-GST
蛋白编号

Q15349 (M1-L733)
基因 ID
蛋白结构
N-term
GST
RSK3 (M1-L733)
Accession # Q15349
C-term
同用名
Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; p90-RSK 2; RSK-3; RPS6KA2; MAPKAPK1C
分子量

Approximately 110 kDa
纯度

Greater than 84% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

RSK3 Protein, Human (sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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