RuvA 蛋白, E.coli (His-SUMO)

RuvA protein is a core component of the RuvA-RuvB-RuvC complex and plays a key role in gene recombination and DNA repair, especially in the processing of Holliday junction (HJ) DNA. RuvA cooperates with RuvB to promote HJ branch migration, binds to HJ cruciform DNA and imparts an open structure. RuvA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RuvA protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of RuvA Protein, E.coli (His-SUMO) is 203 a.a., with molecular weight of ~38.1 kDa.

RuvA 蛋白作为 RuvA-RuvB-RuvC 复合体的关键成分，在基因重组和 DNA 修复中发挥着核心作用，该复合体在这些关键的细胞过程中处理霍利迪连接体 (HJ) DNA。该复合物与 RuvA-RuvB 系统一起，通过复制叉逆转 (RFR) 对挽救受阻的 DNA 复制叉做出了重大贡献，其中 RFR 和同源重组对于紫外线生存至关重要。RuvA 与 RuvB 结合，通过结合 HJ 十字形 DNA 并赋予开放结构，促进 HJ 分支迁移。在存在 RuvB、ATP 和 Mg(2+) 的情况下，连接点会解离，RuvB 六聚体充当分子泵，将双链 DNA 拉入并穿过 RuvAB 复合物。RuvA 通过与十字形连接结合而赋予特异性，而 RuvB 的 ATP 酶活性为分支迁移提供动力，即使在没有 RuvA 的情况下，这一过程也可能发生。单独过度表达 RuvA 会导致紫外线敏感性。此外，RuvA 在 DNA 存在的情况下刺激 RuvB 的弱 ATPase 活性，并且 HJ DNA 的添加进一步增强了 ATPase 活性。RuvA 通过与 HJ DNA 结合来抑制 RuvC 内切 DNA 酶活性，并且它可能充当在 HJ 上滑动的项圈，促进分支迁移，同时抑制其他 DNA 重塑活动。RuvA 在 RuvABC 复合体中的这种全面相互作用强调了其在协调 DNA 修复和重组事件中的多方面功能。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

N-SUMO;N-6*His

P0A809 (M1-L203)

946369  [NCBI]

MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQEAIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL

Approximately 38.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.