S100A8-S100A9 Heterodimer 蛋白, Human (His)

S100A8, binding to calcium and zinc, crucially regulates inflammation and immune responses. As calprotectin, it aids leukocyte functions, modulates cytoskeleton, and activates NADPH-oxidase intracellularly. Extracellularly, it displays pro-inflammatory, antimicrobial, and apoptosis-inducing activities, acting as a DAMP molecule that stimulates innate immune cells through TLR4 and AGER. With antimicrobial effects, it regulates neutrophils, induces cell death, and prevents tissue damage. Notably, it amplifies inflammation in autoimmunity and cancer, potentially influencing aberrant neutrophil expansion in microbial infections like SARS-CoV-2. S100A8-S100A9 Heterodimer Protein, Human (His) is a recombinant protein dimer complex containing human-derived S100A8-S100A9 Heterodimer protein, expressed by E. coli, with C-6*His labeled tag. S100A8-S100A9 Heterodimer Protein, Human (His), has molecular weight of 13 & 15 kDa, respectively.

S100A8 在调节炎症过程和免疫反应中发挥着至关重要的作用。通常以钙卫蛋白 (S100A8/A9) 形式存在，它具有多种细胞内功能，包括促进白细胞花生四烯酸运输和代谢、在吞噬细胞迁移过程中调节微管蛋白依赖性细胞骨架以及激活中性粒细胞 NADPH 氧化酶。特别是，它通过帮助酶复合物在细胞膜上组装、转移花生四烯酸并直接与 NCF2/P67PHOX 结合来激活 NADPH 氧化酶。在细胞外，它表现出促炎、抗菌、清除氧化剂和诱导细胞凋亡的活性。作为警报素或危险相关分子模式 (DAMP) 分子，S100A8 通过与 Toll 样受体 4 (TLR4) 和晚期糖基化终末产物受体 (AGER) 等模式识别受体结合来刺激先天免疫细胞，从而激活 MAP 激酶和 NF-kappa-B 信号通路并放大促炎级联反应。它具有针对细菌和真菌的抗菌活性，很可能通过微生物生长所必需的 Zn(2+) 螯合作用发挥这种作用。此外，S100A8/A9 通过自噬和细胞凋亡诱导细胞死亡，调节中性粒细胞数量和细胞凋亡，并充当氧化剂清除剂以防止组织损伤。它在自身免疫和癌症发展中作为炎症放大器的作用是值得注意的，并且在微生物感染（例如 SARS-CoV-2）中，它可能以 TLR4 依赖性方式诱导异常未成熟中性粒细胞的扩增。

Measured by its ability to induce IL-6 secretion by human melanoma cells. The ED₅₀ for this effect is 1.472 μg/mL, corresponding to a specific activity is 679.348 U/mg.

Human

E. coli

C-6*His

P05109 (M1-E93)(S100A8) & P06702 (T2-P114) (S100A9)

6279  [NCBI]&6280  [NCBI]

A1:MLTELEKALN
SIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE
A2:TCKMSQLERN
IETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP

13&15 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 10% Glycerol, 150 mM NaCl, 2.5 mM EDTA, pH 7.4 or 50 mM HEPES, 300 mM NaCl, 200 mM Arginine, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.