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  3. Semaphorin-3A/SEMA3A 蛋白, Mouse (HEK293, His)

Semaphorin-3A/SEMA3A 蛋白, Mouse (HEK293, His)

目录号: HY-P78350
COA 产品使用指南

Semaphorin-3A/SEMA3A 蛋白引导生长锥并参与感觉投射模式,选择性排斥终止于背侧的轴突。它有助于嗅觉系统的发育和青春期的神经元控制。SEMA3A 与 PXND1 相互作用,但这种相互作用的确切性质尚未明确。Semaphorin-3A/SEMA3A 蛋白, Mouse (HEK293, His) 是重组的 Semaphorin-3A/SEMA3A 蛋白,由 HEK293 表达,带有 N-His 标签。Semaphorin-3A/SEMA3A 蛋白, Mouse (HEK293, His) 全长 752 个氨基酸,分子量约为 ~86.6 kDa。

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规格 价格 是否有货 数量
20 μg ¥1830 In-stock
50 μg ¥4020 In-stock
100 μg   询价  

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描述

Semaphorin-3A/SEMA3A Protein guides growth cones and is implicated in sensory projection patterning, selectively repelling axons that terminate dorsally. It contributes to olfactory system development and neuronal control of puberty. SEMA3A interacts with PXND1, although the exact nature of this interaction is unspecified. Semaphorin-3A/SEMA3A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-3A/SEMA3A protein, expressed by HEK293, with N-His labeled tag. The total length of Semaphorin-3A/SEMA3A Protein, Mouse (HEK293, His) is 752 a.a., with molecular weight of ~86.6 kDa.

研究背景

Semaphorin-3A/SEMA3A 蛋白在引导生长锥中发挥着至关重要的作用。据信,它通过选择性地排斥通常终止于背侧的轴突来参与感觉投射的模式化。SEMA3A 还参与嗅觉系统的发育,并有助于青春期的神经元控制。此外,它与 PXND1 相互作用,尽管这种相互作用的确切性质尚未明确。

种属

Mouse

表达系统

HEK293

标签

N-His

蛋白编号

O08665 (Y21-V772)

基因 ID

20346  [NCBI]

蛋白结构
N-term
His
SEMA3A (Y21-V772)
Accession # O08665
C-term
同用名
Semaphorin-3A; Semaphorin III; Sema III; Semaphorin-D; Sema D; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; SEMA1, Sema3A; SEMAD; SEMAIII; SEMAL; semaphorin L
分子量

Approximately 86.6 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 100 mM L-Arginine, 100 mM glutamic acid, pH 8.5. Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Semaphorin-3A/SEMA3A Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Semaphorin-3A/SEMA3A Protein, Mouse (HEK293, His)
目录号:
HY-P78350
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