Semaphorin-7A/SEMA7A 蛋白, Human (HEK293, His)

Semaphorin-7A (SEMA7A) plays a key role in integrin-mediated signaling, affecting multiple cellular functions, including cell migration and immune responses. SEMA7A promotes focal adhesion complex formation, activates PTK2/FAK1 and induces phosphorylation of MAPK1 and MAPK3. Semaphorin-7A/SEMA7A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-7A/SEMA7A protein, expressed by HEK293 , with C-His labeled tag. The total length of Semaphorin-7A/SEMA7A Protein, Human (HEK293, His) is 604 a.a., with molecular weight of 70-75 kDa.

Semaphorin-7A (SEMA7A) 蛋白在整合素介导的信号传导中发挥着至关重要的作用，对多种细胞功能产生影响，包括细胞迁移和免疫反应的调节。SEMA7A 积极促进粘着斑复合物的形成，激活蛋白激酶 PTK2/FAK1，随后诱导 MAPK1 和 MAPK3 磷酸化。此外，它在协调单核细胞和巨噬细胞产生促炎细胞因子方面发挥着重要作用，从而调节炎症和 T 细胞介导的免疫反应。在发育过程中，SEMA7A 参与促进胚胎嗅球中的轴突生长，并促进黑素细胞中的附着、扩散和树突生长。其复杂的分子相互作用涉及与整合素 ITGA1 和 ITGB1 的关联，表明其参与介导细胞粘附，并且它与 PLXNC1 相互作用，增加了其在多种细胞过程中调节作用的复杂性。

Human

HEK293

C-His

O75326 (Q45-A648)

8482  [NCBI]

70-75 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.