Serpin A5 蛋白, Human (HEK293, His)

Plasma serine protease inhibitor (SERPINA5) is a glycoproteins that inhibit serine proteases. SERPINA5 is involved in the regulation of intravascular and extravascular proteolytic activities, controls the sperm motility and fertilization and protect components of the genital tract. SERPINA5 also inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. Serpin A5 Protein, Human (HEK293, His) is the recombinant human-derived Serpin A5 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin A5 Protein, Human (HEK293, His) is 387 a.a., with molecular weight of 44-55 kDa.

血浆丝氨酸蛋白酶抑制剂 (SERPINA5) 是丝氨酸蛋白酶家族蛋白的一员，丝氨酸蛋白酶是一组抑制丝氨酸蛋白酶的糖蛋白。SERPINA5 是一种肝素依赖性丝氨酸蛋白酶抑制剂，作用于体液和分泌物中，通过不可逆地结合丝氨酸活化位点使丝氨酸蛋白酶失活。
SERPINA5 双模式地调节血管内外的蛋白水解活性。SERPINA5 在血浆中可起止血作用，通过抑制抗凝活性蛋白 C 因子以及凝血酶/凝血调节蛋白复合物生成活化蛋白 C 因子而发挥促凝和促炎因子的作用。此外，SERPINA5 也可以作为一种抗凝因子，通过抑制凝血酶原、因子XI、因子Xa、血浆钾化管等凝血因子和组织型和尿型纤溶酶原激活剂等纤溶酶。
在精浆中，SERPINA5 通过抑制丝氨酸顶体蛋白来间接保护男性生殖道成分不被过量释放的顶体降解，从而使与生殖系统有关的几种丝氨酸蛋白酶失活、抑制组织型和尿型纤溶酶原激活物、前列腺特异性抗原和钾激肽活性、对精子活力和受精有控制作用、抑制活化蛋白 C 催化的 SEMG1 和 SEMG2 降解、在精子从雄性生殖道转移到雌性生殖道的过程中，调节其凝胶的降解。
在尿液中，SERPINA5 抑制尿型纤溶酶原激活物和钾激肽活性。SERPINA5 使膜锚定丝氨酸蛋白酶如MPRSS7 和 TMPRSS11E 失活;抑制尿型纤溶酶原激活物依赖性肿瘤细胞的侵袭和转移，SERPINA5 也可能通过与视黄酸结合，作为疏水激素载体在精浆和尿液中发挥非抑制作用^[1][2][3]。

Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is 1.91 nM.

Human

HEK293

C-6*His

AAH08915.1 (H20-P406)

5104  [NCBI]

HRHHPREMKKRVEDLHVGATVAPSSRRDFTFDLYRALASAAPSQNIFFSPVSISMSLAMLSLGAGSSTKMQILEGLGLNLQKSSEKELHRGFQQLLQELNQPRDGFQLSLGNALFTDLVVDLQDTFVSAMKTLYLADTFPTNFRDSAGAMKQINDYVAKQTKGKIVDLLKNLDSNAVVIMVNYIFFKAKWETSFNHKGTQEQDFYVTSETVVRVPMMSREDQYHYLLDRNLSCRVVGVPYQGNATALFILPSEGKMQQVENGLSEKTLRKWLKMFKKRQLELYLPKFSIEGSYQLEKVLPSLGISNVFTSHADLSGISNHSNIQVSEMVHKAVVEVDESGTRAAAATGTIFTFRSARLNSQRLVFNRPFLMFIVDNNILFLGKVNRP

44-55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.