SIGIRR 蛋白, Human (HEK293, Fc)

SIGIRR Protein functions as a negative regulator of Toll-like and IL-1R receptor signaling pathways. It inhibits recruitment of signaling components to TLR4 receptor through TIR domain interaction. SIGIRR also hinders heterodimerization of Il1R1 and IL1RAP via its extracellular domain. It interacts with IL1R1, IRAK1, TLR4, TLR5, TLR9, TRAF6, and PALM3. Upon IL-1 stimulation, SIGIRR forms complex with IL1R1, SIGIRR, MYD88, IRAK1, and TRAF6. Similarly, upon LPC stimulation, SIGIRR is part of complex including TLR4, SIGIRR, MYD88, IRAK1, and TRAF6. SIGIRR Protein, Human (HEK293, Fc) is the recombinant human-derived SIGIRR protein, expressed by HEK293, with C-hFc labeled tag. The total length of SIGIRR Protein, Human (HEK293, Fc) is 118 a.a., with molecular weight of 50-70 kDa.

SIGIRR 蛋白充当 Toll 样和 IL-1R 受体信号通路的负调节因子。它通过抑制信号成分向 TLR4 受体的募集发挥作用，可能是通过 TIR 结构域之间的相互作用。此外，SIGIRR 通过其胞外结构域干扰 Il1R1 和 IL1RAP 的异二聚化。它与多种蛋白质相互作用，包括 IL1R1、IRAK1、TLR4、TLR5、TLR9、TRAF6 和 PALM3。IL-1 刺激后，可以在由 IL1R1、SIGIRR、MYD88、IRAK1 和 TRAF6 组成的复合物中发现 SIGIRR。类似地，在用 LPC 刺激后，SIGIRR 存在于包含 TLR4、SIGIRR、MYD88、IRAK1 和 TRAF6 的复合物中。

Human

HEK293

C-hFc

Q6IA17 (M1-H118)

59307  [NCBI]

50-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.