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SLC25A19 蛋白, Human (Cell-Free, His)

目录号: HY-P702438
产品使用指南

SLC25A19 是一种关键的线粒体转运蛋白,可促进二磷酸硫胺素摄取进入线粒体。虽然其反向转运蛋白活性对膜电位或质子电化学梯度影响的具体情况尚不清楚,但 SLC25A19 作为转运蛋白的作用强调了其在线粒体过程中的重要性,特别是二磷酸硫胺素(多种代谢途径中的重要辅酶)的转运。SLC25A19 蛋白, Human (Cell-Free, His) 是重组的 SLC25A19 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。SLC25A19 蛋白, Human (Cell-Free, His) 全长 320 个氨基酸,分子量为 37.0 kDa。

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描述

SLC25A19, a pivotal mitochondrial transporter, facilitates thiamine diphosphate uptake into mitochondria. While the specifics of its antiporter activity regarding the influence of membrane potential or proton electrochemical gradient remain unclear, SLC25A19's role as a transporter underscores its importance in mitochondrial processes, particularly the transport of thiamine diphosphate, a vital coenzyme in various metabolic pathways. SLC25A19 Protein, Human (Cell-Free, His) is the recombinant human-derived SLC25A19 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of SLC25A19 Protein, Human (Cell-Free, His) is 320 a.a., with molecular weight of 37.0 kDa.

研究背景

SLC25A19 是一种线粒体转运蛋白,在促进二磷酸硫胺素摄取进入线粒体方面发挥着至关重要的作用。其反向转运蛋白活性的机制仍不清楚,因为尚不清楚这种活性是否受到膜电位或线粒体内质子电化学梯度的影响。尽管如此,SLC25A19 作为转运蛋白的功能凸显了其在线粒体过程中的重要性,特别是在二磷酸硫胺素(一种参与各种代谢途径的重要辅酶)的转运中。

种属

Human

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

Q9HC21 (M1-R320)

基因 ID

60386

蛋白结构
N-term
10*His
SLC25A19 (M1-R320)
Accession # Q9HC21
C-term
同用名
Mitochondrial thiamine pyrophosphate carrier; Mitochondrial thiamine pyrophosphate transporter; MTPPT; Mitochondrial uncoupling protein 1; Solute carrier family 25 member 19
氨基酸序列

MVGYDPKPDGRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVDVLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHLYKWAIPAEGKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALGFFKGLSPSLLKAALSTGFMFFSYEFFCNVFHCMNRTASQR

分子量

37.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SLC25A19 Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SLC25A19 Protein, Human (Cell-Free, His)
目录号:
HY-P702438
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