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  3. SLC30A8 蛋白, Mouse (Cell-Free, His)

SLC30A8 蛋白, Mouse (Cell-Free, His)

目录号: HY-P702442
产品使用指南

SLC30A8 蛋白是一种质子偶联的锌离子反向转运蛋白,对胰岛素分泌起着至关重要的调节作用。作为介质,SLC30A8 使锌能够进入胰腺 β 细胞分泌颗粒,维持所需的锌浓度。这一过程微调胰岛素释放动力学,强调 SLC30A8 在生理胰岛素分泌控制和葡萄糖稳态中的关键作用。SLC30A8 蛋白, Mouse (Cell-Free, His) 是重组的 SLC30A8 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。SLC30A8 蛋白, Mouse (Cell-Free, His) 全长 367 个氨基酸,分子量为 43.0 kDa。

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描述

SLC30A8 Protein, a proton-coupled zinc ion antiporter, crucially regulates insulin secretion. Serving as a mediator, SLC30A8 enables zinc entry into pancreatic beta cell secretory granules, maintaining the required zinc concentration. This process finely tunes insulin release dynamics, emphasizing SLC30A8's pivotal role in physiological insulin secretion control and glucose homeostasis. SLC30A8 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived SLC30A8 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of SLC30A8 Protein, Mouse (Cell-Free, His) is 367 a.a., with molecular weight of 43.0 kDa.

研究背景

SLC30A8 蛋白被鉴定为质子偶联锌离子反向转运蛋白,在胰岛素分泌的复杂调节中发挥着关键作用。SLC30A8 作为介质发挥作用,促进锌进入胰腺 β 细胞分泌颗粒的管腔。这个过程对于维持颗粒内适当的锌浓度至关重要,最终影响胰岛素释放的动态。通过参与锌的转运,SLC30A8 有助于精细协调胰腺 β 细胞内的细胞事件,突显其在胰岛素分泌的生理控制以及葡萄糖稳态中的重要性。

种属

Mouse

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

Q8BGG0 (M1-D367)

基因 ID

239436

蛋白结构
N-term
10*His
SLC30A8 (M1-D367)
Accession # Q8BGG0
C-term
同用名
Proton-coupled zinc antiporter SLC30A8; Solute carrier family 30 member 8; Zinc transporter 8; ZnT-8
氨基酸序列

MEFLERTYLVNDQATKMYAFPLDRELRQKPVNKDQCPGDRPEHPEAGGIYHCHNSAKATGNRSSKQAHAKWRLCAASAICFIFMVAEVVGGHVAGSLAILTDAAHLLIDLTSFLLSLFSLWLSSRPPSKRLTFGWYRAEILGALLSVLCIWVVTGVLLYLACERLLYPDYQIQAGIMITVSGCAVAANIVLTMILHQRNFGYNHKDVQANASVRAAFVHALGDVFQSISVLISALIIYFKPDYKIADPVCTFIFSILVLASTVMILKDFSILLMEGVPKGLSYNSVKEIILAVDGVISVHSLHIWSLTVNQVILSVHVATAASQDSQSVRTGIAQALSSFDLHSLTIQIESAADQDPSCLLCEDPQD

分子量

43.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SLC30A8 Protein, Mouse (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SLC30A8 Protein, Mouse (Cell-Free, His)
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HY-P702442
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