Stathmin 蛋白, Human (C-His)

Stathmin proteins critically regulate the microtubule filament system by actively destabilizing microtubules, inhibiting assembly, and promoting disassembly. Phosphorylation of Ser-16 is critical for axon formation during neurogenesis, highlighting its importance in neuronal development. Stathmin Protein, Human (C-His) is the recombinant human-derived Stathmin protein, expressed by E. coli , with C-6*His labeled tag. The total length of Stathmin Protein, Human (C-His) is 148 a.a., with molecular weight of ~20.0 kDa.

Stathmin 蛋白通过主动破坏微管的稳定性，从而防止其组装并促进解体，从而在调节微管丝系统中发挥至关重要的作用。Stathmin Ser-16 的磷酸化可能是神经发生过程中轴突形成的关键要求，强调了其在神经元发育中的重要性。除了在细胞结构中的作用之外，Stathmin 还涉及后天恐惧和先天恐惧的控制，这表明它可能参与复杂的行为过程。该蛋白与两个 α/β-微管蛋白异二聚体结合，形成对其微管调节功能至关重要的相互作用。此外，Stathmin 与 KIST 相互作用，进一步扩展其分子关联网络，并表明其参与多种细胞过程。

Human

E. coli

C-6*His

P16949 (A2-D149)

3925  [NCBI]

ASSDIQVKELEKRASGQAFELILSPRSKESVPEFPLSPPKKKDLSLEEIQKKLEAAEERRKSHEAEVLKQLAEKREHEKEVLQKAIEENNNFSKMAEEKLTHKMEANKENREAQMAAKLERLREKDKHIEEVRKNKESKDPADETEAD

Approximately 20.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.