1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. STEAP1 蛋白, Mouse (Cell-Free, His)

STEAP1 蛋白, Mouse (Cell-Free, His)

目录号: HY-P702448
产品使用指南

STEAP1蛋白被归类为金属还原酶家族的成员,不能作为功能性金属还原酶发挥作用,这主要是由于缺乏给电子底物NADPH的结合位点。这一独特的功能将 STEAP1 与典型的金属还原酶区分开来,后者的特点是能够使用 NADPH 作为电子供体催化金属离子的还原。STEAP1 蛋白, Mouse (Cell-Free, His) 是重组的 STEAP1 蛋白,由 E. coli Cell-free 表达,带有 C-10*His 标签。STEAP1 蛋白, Mouse (Cell-Free, His) 全长 339 个氨基酸,分子量为 40.7 kDa。

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描述

The STEAP1 protein is classified as a member of the metal reductase family and cannot function as a functional metal reductase, mainly due to the lack of binding sites for the electron-donating substrate NADPH. This unique feature distinguishes STEAP1 from typical metal reductases, which are characterized by their ability to catalyze the reduction of metal ions using NADPH as an electron donor. STEAP1 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived STEAP1 protein, expressed by E. coli Cell-free , with C-10*His labeled tag. The total length of STEAP1 Protein, Mouse (Cell-Free, His) is 339 a.a., with molecular weight of 40.7 kDa.

研究背景

据现有信息表明,STEAP1(前列腺六跨膜上皮抗原 1)蛋白不能作为金属还原酶起作用,主要是因为它缺乏供电子底物 NADPH 的结合位点。金属还原酶通过还原金属离子在细胞过程中发挥着至关重要的作用,但 STEAP1 中 NADPH 结合位点的缺失表明其具有独特的功能特征。这一观察结果强调了了解 STEAP1 的结构和生化特征对于阐明其在细胞通路中的作用的重要性。需要进一步的研究来揭示与 STEAP1 相关的特定功能和影响,无论是在正常细胞过程中还是与疾病或生理条件的潜在联系中,因为它的独特特征可能有助于其在细胞环境中发挥独特的作用。(参考资料:根据现有资料改编,未注明具体出处)

种属

Mouse

表达系统

E. coli Cell-free

标签

C-10*His

蛋白编号

Q9CWR7 (M1-L339)

基因 ID

70358

蛋白结构
N-term
STEAP1 (M1-L339)
Accession # Q9CWR7
10*His
C-term
同用名
Metalloreductase STEAP1; Six-transmembrane epithelial antigen of prostate 1
氨基酸序列

MEISDDVTNPEQLWKMKPKGNLEDDSYSTKDSGETSMLKRPGLSHLQHAVHVDAFDCPSELQHTQEFFPNWRLPVKVAAIISSLTFLYTLLREIIYPLVTSREQYFYKIPILVINKVLPMVAITLLALVYLPGELAAVVQLRNGTKYKKFPPWLDRWMLARKQFGLLSFFFAVLHAVYSLSYPMRRSYRYKLLNWAYKQVQQNKEDAWVEHDVWRMEIYVSLGIVGLAILALLAVTSIPSVSDSLTWREFHYIQSKLGIVSLLLGTVHALVFAWNKWVDVSQFVWYMPPTFMIAVFLPTLVLICKIALCLPCLRKKILKIRCGWEDVSKINRTEMASRL

分子量

40.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

STEAP1 Protein, Mouse (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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STEAP1 Protein, Mouse (Cell-Free, His)
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