STIM1 蛋白, Human (HEK293, His)

STIM1 (stromal interacting molecule 1) coordinates store-operated Ca(2+) entry (SOCE) and acts as a Ca(2+) sensor in the endoplasmic reticulum. Upon Ca(2+) depletion, STIM1 translocates to the plasma membrane and activates the Ca(2+)-releasing activated Ca(2+) (CRAC) channel subunit ORAI1. STIM1 Protein, Human (HEK293, His) is the recombinant human-derived STIM1 protein, expressed by HEK293 , with C-His labeled tag. The total length of STIM1 Protein, Human (HEK293, His) is 191 a.a., with molecular weight of 27-34 kDa.

STIM1（基质相互作用分子 1）在介导钙库操纵的 Ca(2+) 进入 (SOCE) 中发挥关键作用，该过程涉及细胞内 Ca(2+) 储备耗尽后 Ca(2+) 流入。STIM1 通过其 EF-hand 结构域在内质网中充当 Ca(2+) 传感器，在 Ca(2+) 耗尽时从内质网易位到质膜。在此激活状态下，它与 Ca(2+) 释放激活的 Ca(2+) (CRAC) 通道亚基 ORAI1 相互作用并激活。STIM1 与 STIM2 形成同源寡聚体和异源寡聚体，其与其他蛋白（如 SPPL3、MAPRE1、CRACR2A/EFCAB4B、SARAF、EFHB、ASPH、SLC35G1、TMEM203、CASQ1 和 ADCY8）的相互作用进一步调节其细胞功能，包括 SOCE 活性。此外，STIM1 参与牙釉质形成，并通过与 STIMATE 相互作用促进构象变化。这些动态相互作用凸显了 STIM1 在细胞 Ca(2+) 信号传导和稳态中的多方面作用。

Data is not available.

Human

HEK293

C-His

Q13586/NP_003147.2(L23-D213)

6786  [NCBI]

LSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKD

27-34 kDa. The protein migrates as 27-34 kDa under reducing SDS-PAGE due to glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.