1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. THOP1 蛋白, Mouse (sf9, His)

THOP1 蛋白, Mouse (sf9, His)

目录号: HY-P77230
产品使用指南

THOP1蛋白在少于20个氨基酸的神经肽代谢中起关键作用,并参与细胞质肽的降解。除了一般的肽代谢之外,THOP1 还能降解淀粉样β前体蛋白,使其与神经退行性疾病有关。THOP1 蛋白, Mouse (sf9, His) 是重组的 THOP1 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。THOP1 蛋白, Mouse (sf9, His) 全长 686 个氨基酸,分子量约为 ~75 kDa。

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     可免费申领三个不同产品的试用装。

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描述

THOP1 protein plays a key role in the metabolism of neuropeptides with less than 20 amino acids and participates in the degradation of cytoplasmic peptides. In addition to general peptide metabolism, THOP1 degrades amyloid beta precursor protein, implicating it in neurodegenerative diseases. THOP1 Protein, Mouse (sf9, His) is the recombinant mouse-derived THOP1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of THOP1 Protein, Mouse (sf9, His) is 686 a.a., with molecular weight of ~75 kDa.

研究背景

THOP1 蛋白在 20 个氨基酸残基以下的神经肽代谢中发挥着至关重要的作用,参与细胞质肽降解。除了参与一般肽代谢之外,THOP1 还具有降解淀粉样β前体蛋白、产生淀粉样蛋白片段并使其参与与神经退行性疾病相关的过程的能力。此外,THOP1 通过介导升压素(大麻素受体 CNR1 的拮抗剂肽)的降解,充当大麻素信号通路的调节剂。这些不同的功能凸显了 THOP1 在复杂的细胞过程网络中的重要性,从神经肽代谢到信号通路中的调节作用,对神经系统和信号相关疾病具有潜在影响。

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

Q8C1A5 (K2-C687)

基因 ID

50492  [NCBI]

蛋白结构
N-term
His
THOP1 (K2-C687)
Accession # Q8C1A5
C-term
同用名
Thimet Oligopeptidase; Endopeptidase 24.15; MP78; THOP1
分子量

Approximately 75 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

THOP1 Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
THOP1 Protein, Mouse (sf9, His)
目录号:
HY-P77230
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