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  4. TPK1 蛋白, Human (HEK293, His)

TPK1 蛋白, Human (HEK293, His)

目录号: HY-P71376
产品使用指南

硫胺素焦磷酸激酶1 (TPK1) 作为一种同二聚体，催化硫胺素 (维生素B1) 转化为焦磷酸硫胺素 (TDP)，一个糖酵解和能量产生途径中一些酶的辅助因子和醛基的瞬时中间载体。TPK1 还可以催化吡硫胺磷酸化为吡硫胺焦磷酸。TPK1 蛋白, Human (HEK293, His) 是重组的 TPK1 蛋白，由 HEK293 表达，带有 C-6*His 标签。TPK1 蛋白, Human (HEK293, His) 全长 243 个氨基酸，分子量约为 ~30.0 kDa。

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描述

Thiamin pyrophosphokinase 1 (TPK1) functions as a homodimer and catalyzes the conversion of thiamine (Vitamin B1) to thiamine pyrophosphate (TDP), a cofactor and a transient intermediate carrier of the aldehyde group for some enzymes of the glycolytic and energy production pathways. TPK1 can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. TPK1 Protein, Human (HEK293, His) is the recombinant human-derived TPK1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TPK1 Protein, Human (HEK293, His) is 243 a.a., with molecular weight of ~30.0 kDa.
研究背景

硫胺素焦磷酸激酶1 (TPK1) 作为一种同二聚体，催化硫胺素 (维生素B1) 转化为焦磷酸硫胺素 (TDP)，一个糖酵解和能量产生途径中一些酶的辅助因子。在丙酮酸脱氢酶、2-氧戊二酸脱氢酶、转酮醇酶等依赖于 TDP 的酶的反应中，TDP 作为醛基的瞬时中间载体。TPK1 还可以催化吡硫胺磷酸化为吡硫胺焦磷酸。TPK1 基因缺陷是导致硫胺素代谢功能障碍综合征-5 的原因之一[1]。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

AAH68460.1 (M1-S243)
基因 ID
蛋白结构
N-term
TPK1 (M1-S243)
Accession # AAH68460.1
6*His
C-term
同用名
Thiamin pyrophosphokinase 1; hTPK1; Placental protein 20; PP20; Thiamine pyrophosphokinase 1; TPK1
氨基酸序列

MEHAFTPLEPLLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCSQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS

分子量

Approximately 30.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

TPK1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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