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  3. TsaE 蛋白, E.coli

TsaE 蛋白, E.coli

目录号: HY-P71500
产品使用指南

TsaE 蛋白对于在解码腺嘌呤起始密码子的 tRNA 中腺苷位置 37 (t(6)A37) 处生成苏氨酰氨基甲酰基至关重要。它可以促进苏氨甲酰基部分从苏氨甲酰基-AMP (TC-AMP) 转移到 A37 的 N6 基团,与 TsaD 和 TsaB 协同作用。TsaE 蛋白, E.coli 是重组的 TsaE 蛋白,由 E. coli 表达,不带标签。TsaE 蛋白, E.coli 全长 153 个氨基酸,分子量约为 ~16.9 kDa。

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描述

The TsaE protein is essential for the generation of the threonylcarbamoyl group at adenosine position 37 (t(6)A37) in the tRNA that decodes the adenine start codon. It may facilitate the transfer of the threoncarbamoyl moiety from threoncarbamoyl-AMP (TC-AMP) to the N6 group of A37, acting synergistically with TsaD and TsaB. TsaE Protein, E.coli is the recombinant E. coli-derived TsaE protein, expressed by E. coli , with tag free. The total length of TsaE Protein, E.coli is 153 a.a., with molecular weight of ~16.9 kDa.

研究背景

TsaE 是 tRNA 修饰复杂过程中的关键因素,特别是在 tRNA 中 37 位腺苷 (t(6)A37) 上形成苏氨酰氨基甲酰基团的过程中,该 tRNA 解码以腺嘌呤开头的密码子。提议其与 TsaD 和 TsaB 合作,参与促进苏氨酰氨基甲酰基部分从苏氨酰氨基甲酰基-AMP (TC-AMP) 转移到 A37 的 N6 基团。虽然 TsaE 在 t(6)A 生物合成途径中的确切作用仍不清楚,但建议发挥间接影响,可能调节 TsaD 的核心酶功能。有趣的是,TsaE 在体外展示了 ATP 酶活性,强调了它在对 tRNA 修饰至关重要的分子过程中的动态参与。

种属

E.coli

表达系统

E. coli

标签

Tag Free

蛋白编号

P0AF67 (1M-153G)

基因 ID

60902986

蛋白结构
N-term
TsaE (1M-153G)
Accession # P0AF67
C-term
同用名
tsaE; yjeE; b4168; JW4126tRNA threonylcarbamoyladenosine biosynthesis protein TsaE; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
氨基酸序列

MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG

分子量

Approximately 16.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TsaE Protein, E.coli 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TsaE Protein, E.coli
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HY-P71500
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