VEGF-A 蛋白, Pig (His)

The VEGF-A protein is a multifunctional growth factor that is essential for promoting angiogenesis, vasculogenesis, and endothelial cell growth. Its multiple effects include inducing endothelial cell proliferation, promoting migration, inhibiting apoptosis, and increasing vascular permeability. VEGF-A Protein, Pig (His) is the recombinant pig-derived VEGF-A protein, expressed by E. coli , with N-6*His labeled tag. The total length of VEGF-A Protein, Pig (His) is 164 a.a., with homodimer molecular weight of ~23.2 kDa.

VEGF-A 蛋白是一种多功能生长因子，在血管生成、血管生成和内皮细胞生长中发挥着至关重要的作用。其多种作用包括诱导内皮细胞增殖、促进细胞迁移、抑制细胞凋亡和诱导血管通透化。VEGF-A 通过与受体 FLT1/VEGFR1 和 KDR/VEGFR2 以及硫酸乙酰肝素和肝素结合来实现这些功能。值得注意的是，它与 NRP1 受体的相互作用启动了胚胎发育过程中运动神经元轴突引导和细胞体迁移所必需的信号通路。此外，VEGF-A 与 DEAR/FBXW7-AS1 受体结合，进一步扩大其调节作用。在结构上，VEGF-A以二硫键连接的同二聚体形式存在，也可以与PGF形成异二聚体。与 NRP1 和 BSG 的相互作用强调了 VEGF-A 在协调血管过程和胚胎发育中的多方面功能背后的复杂分子关系。

Pig

E. coli

N-6*His

P49151 (A27-R190)

397157  [NCBI]

APMAEGDQKPHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEEFNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Homodimer. Approximately 23.2kDa on SDS-PAGE under reducing conditions

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.