VEGF164 蛋白, Mouse (186a.a, P.pastoris)

VEGF164 Protein, Mouse (186a.a, P.pastoris), a VEGF isoform splice variant, is more potent at inducing endothelial ICAM-1 expression by VEGFR-2, leukocyte migration by VEGFR-1, and inflammation and angiogenesis in the adult cornea.

VEGF164被发现在诱导炎症方面更有效。体内阻断 VEGF 受体 (VEGFR)-1 显着抑制 VEGF164 诱导的角膜炎症。在体外，VEGF164 更有效地刺激内皮细胞上的细胞内粘附分子 (ICAM)-1 表达，这是由 VEGFR2 介导的效应。VEGF164 在诱导单核细胞趋化性方面也更有效，这是由 VEGFR1 介导的效应。在永生化人类白细胞系中，我们发现 VEGF164 可以更有效地诱导 VEGFR1 酪氨酸磷酸化^[1]。VEGF164 是早期糖尿病视网膜病变发病机制中的重要亚型^[2]。

The ED₅₀ is <6 ng/mL as measured by HUVEC cells, corresponding to a specific activity of >1.7 × 10⁵ units/mg.

Mouse

P. pastoris

Tag Free

Q00731-2 (A27-R190)

22339  [NCBI]

MAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Approximately 39 kDa (Disulfide-linked homodimer)

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Usui T, et al. VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2. Invest Ophthalmol Vis Sci. 2004 Feb;45(2):368-74.  [Content Brief]

  [2]. Ishida S,et al. VEGF164 is proinflammatory in the diabetic retina. Invest Ophthalmol Vis Sci. 2003 May;44(5):2155-62.  [Content Brief]