VEGF165 蛋白, Human (Biotinylated, HEK293, Avi)

VEGF165 is a member of the PDGF/VEGF family encoding a heparin-binding homodimer that is crucial in inducing vascular endothelial cell proliferation and migration during angiogenesis. The VEGF gene is critical for blood vessel development, and its disruption leads to abnormal blood vessel formation in mouse embryos. VEGF165 Protein, Human (Biotinylated, HEK293, Avi) is the recombinant human-derived VEGF165 protein, expressed by HEK293 , with N-Avi labeled tag. The total length of VEGF165 Protein, Human (Biotinylated, HEK293, Avi) is 165 a.a., with molecular weight of 25-30 kDa.

VEGF165 是 PDGF/VEGF 生长因子家族的成员，编码肝素结合蛋白，形成二硫键连接的同二聚体。这种生长因子在诱导血管内皮细胞增殖和迁移方面发挥着关键作用，对于生理和病理性血管生成都至关重要。小鼠体内 VEGF 基因的破坏导致胚胎血管形成异常，强调了其在血管发育中的重要性。在各种肿瘤中观察到 VEGF 表达升高，与肿瘤分期和进展相关。此外，VEGF 水平升高与 POEMS 综合征、1 型糖尿病微血管并发症 (MVCD1) 和动脉粥样硬化等疾病相关。选择性剪接产生不同的异构体，包括具有抗血管生成特性的 C 末端延伸异构体。在感染严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 期间，VEGF 水平升高，通过募集炎症细胞和增加血管生成素 II (Ang II) 水平促进炎症，从而与 Ang II 建立反馈循环，以抑制炎症反应。持续释放炎症细胞因子。VEGF 在甲状腺、前列腺和其他 21 种组织等组织中的广泛表达强调了它广泛参与多种生理过程。

Measured by its binding ability in a functional ELISA. Immobilized Anti-VEGFA antibody at 5 μg/mL can bind Biotinylated Human VEGF165.The EC₅₀ is 6.080-7.120 ng/mL.

Human

HEK293

N-Avi

NP_001165097 (A27-R191)

7422  [NCBI]

25-30 kDa

Greater than 95% as determined by reduced SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.