VEGF-AA 蛋白, Canine (HEK293)

VEGFA Protein is a vascular endothelial growth factor that is active in angiogenesis and endothelial cell growth. VEGFA Protein induces endothelial cell proliferation, promotes cell migration, inhibits cell apoptosis, and induces vascular permeability. VEGFA Protein consists of 214 amino acids (M1-R214). VEGF-AA Protein, Canine (HEK293) is the recombinant canine-derived VEGF-AA protein, expressed by HEK293 , with tag free. The total length of VEGF-AA Protein, Canine (HEK293) is 190 a.a., with molecular weight of ~23 kDa.

VEGFA Protein在血管生成和内皮细胞生长中具有活性。能诱导内皮细胞增殖，促进细胞迁移，抑制细胞凋亡，诱导血管通透。
VEGFA Protein 能与 FLT1/VEGFR1 和 KDR/VEGFR2 受体、DEAR/FBXW7-AS1 受体、硫酸乙酰肝和肝素结合。
VEGFA Protein 与 NRP1 受体结合启动运动神经元轴突引导和细胞体迁移所需的信号通路，包括胚胎发育过程中面部运动神经元从菱形 4 到菱形 6 的尾部迁移。
VEGF-A Protein 参与了犬肥大细胞瘤 (MCT) 的进展和恶性化^[1][2]。

1.Immobilized canine VEGF164/VEGFA at 10 μg/mL (100 μL/well) can bind human VEGFR2-Fc and the EC₅₀ is 33.83-78.95 ng/mL.
2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED₅₀ for this effect is typically 2-12 ng/mL.

Canine

HEK293

Tag Free

NP_001103972.1 (M1-R190)

403802  [NCBI]

MNFLLSWVHWSLALLLYLHHAKWSQAAPMAGGEHKPHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEEFNITMQIMRIKPHQGQHIGEMSFLQHSKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Approximately 23 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.