Wnt3a 蛋白, Human (His)

Wnt3a protein is a ligand of Frizzled receptors and can activate TCF/LEF transcription factors in the canonical Wnt pathway. It is essential for embryonic mesoderm development, caudal somite formation, neural tube morphogenesis, and intestinal crypt stem cell self-renewal. Wnt3a Protein, Human (His) is the recombinant human-derived Wnt3a protein, expressed by E. coli , with N-6*His labeled tag. The total length of Wnt3a Protein, Human (His) is 333 a.a., with molecular weight of ~40 kDa.

Wnt3a 蛋白作为七跨膜受体卷曲家族成员的配体，可能在经典 Wnt 信号通路中发挥作用，导致 TCF/LEF 家族转录因子的激活。它对于正常胚胎中胚层发育、尾体节形成和发育中的神经管的形态发生至关重要。Wnt3a 还在体外介导肠隐窝基部干细胞的自我更新中发挥作用。蛋白质与AFM形成可溶性1:1复合物，防止寡聚化并确保延长生物活性；该复合物可能代表体液中的生理形式。Wnt3a 通过二硫键形成同源寡聚物，从而导致失活。它与 PORCN 和 Wnt-Fzd-LRP5-LRP6 信号复合物的组件（包括 LRP6、APCDD1 和 WLS）相互作用。此外，Wnt3a 通过其胞外结构域与磷脂酰肌醇蛋白聚糖 GPC3 和 PKD1 相互作用。这些相互作用强调了 Wnt3a 参与各种发育和信号传导过程的复杂性。

Human

E. coli

N-6*His

P56704 (S19-C351)

89780  [NCBI]

SYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTC

Approximately 40 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.