YAP1 蛋白, Human (P.pastoris, His)

YAP1 is a multifunctional transcriptional regulator and an important downstream target in the Hippo signaling pathway, affecting organ size control and tumor suppression through proliferation and apoptosis regulation. STK3/MST2 and STK4/MST1 phosphorylate together with SAV1, inactivating YAP1 and WWTR1/TAZ oncoproteins. YAP1 Protein, Human (P.pastoris, His) is the recombinant human-derived YAP1 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of YAP1 Protein, Human (P.pastoris, His) is 504 a.a., with molecular weight of ~68 kDa.

YAP1 是一种多方面的转录调节因子，是 Hippo 信号通路中的关键下游靶标，通过调节增殖和凋亡来协调器官大小控制和肿瘤抑制。该通路涉及激酶级联反应，其中 STK3/MST2 和 STK4/MST1 与 SAV1 协调，磷酸化并激活 LATS1/2，导致 YAP1 和 WWTR1/TAZ 癌蛋白磷酸化和失活。YAP1 通过 ARHGAP18 影响皮质肌动球蛋白网络的形成，在调节组织张力和 3D 组织形状方面发挥关键作用。此外，它在控制细胞增殖中发挥着关键作用，其核转位受到 LATS1/2 磷酸化的抑制。YAP1 与 TEAD 转录因子的协作对于刺激基因表达、细胞生长、贴壁独立生长和上皮间质转化 (EMT) 诱导至关重要。此外，它通过充当 TEAD4 靶基因 AURKA 和 PLK1 的转录辅阻遏物来抑制纤毛发生，并与 WWTR1 结合，调节 TGFB1 依赖性 SMAD2 和 SMAD3 核积累。YAP1 进一步激活 ERBB4 同工型 3 的 C 末端片段 (CTF)。

Human

P. pastoris

N-6*His

P46937 (M1-L504)

10413  [NCBI]

MDPGQQPPPQPAPQGQGQPPSQPPQGQGPPSGPGQPAPAATQAAPQAPPAGHQIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTPTGVVSGPAATPTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL

Approximately 68 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.