2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. ZRANB1 蛋白, Human (Sf9, His)

ZRANB1 蛋白, Human (Sf9, His)

目录号: HY-P701486
产品使用指南

ZRANB1 是一种泛素硫酯酶，可特异性水解“Lys-29”连接和“Lys-33”连接的双泛素，并对“Lys-63”连接的链具有额外的活性。它通过去泛素化 APC（Wnt 介导的转录抑制剂）来正向调节 Wnt 通路。ZRANB1 蛋白, Human (Sf9, His) 是重组的 ZRANB1 蛋白，由 Sf9 insect cells 表达，带有 C-8*His 标签。ZRANB1 蛋白, Human (Sf9, His) 全长 708 个氨基酸。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

ZRANB1 is a ubiquitin thioesterase that specifically hydrolyzes “Lys-29”-linked and “Lys-33”-linked diubiquitins, with additional activity on “Lys-63”-linked chains. It positively regulates the Wnt pathway by deubiquitinating APC, an inhibitor of Wnt-mediated transcription. ZRANB1 Protein, Human (Sf9, His) is the recombinant human-derived ZRANB1 protein, expressed by Sf9 insect cells , with C-8*His labeled tag. The total length of ZRANB1 Protein, Human (Sf9, His) is 708 a.a., .
研究背景

ZRANB1 作为泛素硫酯酶发挥作用，表现出水解“Lys-29”连接和“Lys-33”连接双泛素的特异性，并且具有裂解“Lys-63”连接链的额外活性，尽管效率较低。值得注意的是，ZRANB1 通过去泛素化 APC 蛋白（Wnt 介导的转录的负调节因子）而充当 Wnt 信号通路的正调节因子。此外，它通过 PIK3C3/VPS34 去泛素化在自噬调节中发挥关键作用，促进自噬体成熟。除了参与细胞内过程之外，ZRANB1 还参与细胞形态和细胞骨架组织的调节，有助于应力纤维动力学和细胞迁移。
种属

Human
表达系统

Sf9 insect cells
标签

C-8*His
蛋白编号

Q9UGI0 (M1-E708)
基因 ID

54764
蛋白结构
N-term
ZRANB1 (M1-E708)
Accession # Q9UGI0
8*His
C-term
同用名
ZRANB1; Ubiquitin thioesterase ZRANB1; TRAF-binding domain-containing protein; hTrabid; Zinc finger Ran-binding domain-containing protein 1
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ZRANB1 Protein, Human (Sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
ZRANB1 Protein, Human (Sf9, His)
目录号：
HY-P701486
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z